{"doi":"10.3390/ijms23137202","title":"Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans","abstract":"The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex.","journal":"International Journal of Molecular Sciences","year":2022,"id":283650,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":960481,"name":"Chiemekam Samuel Ezechukwu","orcid":"0000-0001-8402-0057","position":1,"is_corresponding":false},{"id":960950,"name":"Guiyan Liao","orcid":null,"position":2,"is_corresponding":false},{"id":339170,"name":"Siqiao Li","orcid":"0000-0002-3904-7397","position":3,"is_corresponding":false},{"id":960482,"name":"Zhongliang Chen","orcid":"0000-0002-4129-5787","position":4,"is_corresponding":false},{"id":960483,"name":"Xihui Liu","orcid":"0000-0002-4686-6892","position":5,"is_corresponding":false},{"id":339175,"name":"Wenxia Fang","orcid":"0000-0001-8055-2072","position":6,"is_corresponding":false},{"id":339173,"name":"Cheng Jin","orcid":"0000-0002-1514-6374","position":7,"is_corresponding":false},{"id":339174,"name":"Bin Wang","orcid":"0000-0001-7665-6290","position":8,"is_corresponding":false},{"id":339169,"name":"Arome Solomon Odiba","orcid":"0000-0001-9581-0724","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":null,"created_at":"2026-07-19T00:29:28.452697Z","pmid":"35806213","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}