{"doi":"10.3390/ijms23116080","title":"Immunogenicity of MultiTEP-Platform-Based Recombinant Protein Vaccine, PV-1950R, Targeting Three B-Cell Antigenic Determinants of Pathological α-Synuclein","abstract":"Parkinson's disease (PD) and dementia with Lewy bodies (DLB) are characterized by the aberrant accumulation of intracytoplasmic misfolded and aggregated α-synuclein (α-Syn), resulting in neurodegeneration associated with inflammation. The propagation of α-Syn aggregates from cell to cell is implicated in the spreading of pathological α-Syn in the brain and disease progression. We and others demonstrated that antibodies generated after active and passive vaccinations could inhibit the propagation of pathological α-Syn in the extracellular space and prevent/inhibit disease/s in the relevant animal models. We recently tested the immunogenicity and efficacy of four DNA vaccines on the basis of the universal MultiTEP platform technology in the DLB/PD mouse model. The antibodies generated by these vaccines efficiently reduced/inhibited the accumulation of pathological α-Syn in the different brain regions and improved the motor deficit of immunized female mice. The most immunogenic and preclinically effective vaccine, PV-1950D, targeting three B-cell epitopes of pathological α-Syn simultaneously, has been selected for future IND-enabling studies. However, to ensure therapeutically potent concentrations of α-Syn antibodies in the periphery of the vaccinated elderly, we developed a recombinant protein-based MultiTEP vaccine, PV-1950R/A, and tested its immunogenicity in young and aged D-line mice. Antibody responses induced by immunizations with the PV-1950R/A vaccine and its homologous DNA counterpart, PV-1950D, in a mouse model of PD/DLB have been compared.","journal":"International Journal of Molecular Sciences","year":2022,"id":281415,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":462318,"name":"Gor Chailyan","orcid":null,"position":1,"is_corresponding":false},{"id":462316,"name":"Armine Hovakimyan","orcid":null,"position":2,"is_corresponding":false},{"id":462317,"name":"Tatevik Antonyan","orcid":null,"position":3,"is_corresponding":false},{"id":231734,"name":"Sepideh Kiani Shabestari","orcid":"0000-0002-2840-116X","position":4,"is_corresponding":false},{"id":460774,"name":"Irina Petrushina","orcid":"0000-0001-7313-6943","position":5,"is_corresponding":false},{"id":231744,"name":"Hayk Davtyan","orcid":"0000-0001-8554-6456","position":6,"is_corresponding":false},{"id":460777,"name":"David H. Cribbs","orcid":"0000-0002-6870-4480","position":7,"is_corresponding":false},{"id":7841,"name":"Mathew Blurton-Jones","orcid":"0000-0002-7770-7157","position":8,"is_corresponding":false},{"id":58514,"name":"Eliezer Masliah","orcid":"0000-0002-2117-5569","position":9,"is_corresponding":false},{"id":460779,"name":"Michael G. Agadjanyan","orcid":"0000-0002-9602-0406","position":10,"is_corresponding":false},{"id":460778,"name":"Anahit Ghochikyan","orcid":"0000-0001-5436-0616","position":11,"is_corresponding":false},{"id":456285,"name":"Karen Zagorski","orcid":"0000-0002-6236-0808","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-19T00:29:11.698554Z","pmid":"35682759","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}