{"doi":"10.3390/ijms222413590","title":"Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts","abstract":")-ATPase (PMCA)-Neuroplastin (Np) complexes has renewed attention on cell regulation of cytosolic calcium extrusion, which is of particular relevance in neurons. Here, we tested the hypothesis that PMCA-Neuroplastin complexes exist in specific ganglioside-containing rafts, which could affect calcium homeostasis. We analyzed the abundance of all four PMCA paralogs (PMCA1-4) and Neuroplastin isoforms (Np65 and Np55) in lipid rafts and bulk membrane fractions from GM2/GD2 synthase-deficient mouse brains. In these fractions, we found altered distribution of Np65/Np55 and selected PMCA isoforms, namely PMCA1 and 2. Cell surface staining and confocal microscopy identified GM1 as the main complex ganglioside co-localizing with Neuroplastin in cultured hippocampal neurons. Furthermore, blocking GM1 with a specific antibody resulted in delayed calcium restoration of electrically evoked calcium transients in the soma of hippocampal neurons. The content and composition of all ganglioside species were unchanged in Neuroplastin-deficient mouse brains. Therefore, we conclude that altered composition or disorganization of ganglioside-containing rafts results in changed regulation of calcium signals in neurons. We propose that GM1 could be a key sphingolipid for ensuring proper location of the PMCA-Neuroplastin complexes into rafts in order to participate in the regulation of neuronal calcium homeostasis.","journal":"International Journal of Molecular Sciences","year":2021,"id":171285,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9592,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":705346,"name":"Xiao Lin","orcid":"0000-0002-2891-0263","position":1,"is_corresponding":false},{"id":705347,"name":"Ayse Malci","orcid":"0000-0002-0725-0235","position":2,"is_corresponding":false},{"id":705348,"name":"Mario Stojanović","orcid":"0000-0002-6687-1808","position":3,"is_corresponding":false},{"id":705920,"name":"Borna Puljko","orcid":null,"position":4,"is_corresponding":false},{"id":705349,"name":"Marko Rožman","orcid":"0000-0001-5513-3386","position":5,"is_corresponding":false},{"id":705921,"name":"Željka Vukelić","orcid":null,"position":6,"is_corresponding":false},{"id":705350,"name":"Marija Heffer","orcid":"0000-0001-6770-7359","position":7,"is_corresponding":false},{"id":705351,"name":"Dirk Montag","orcid":"0000-0002-4964-1330","position":8,"is_corresponding":false},{"id":483642,"name":"Ronald L. Schnaar","orcid":"0000-0002-7701-5484","position":9,"is_corresponding":false},{"id":705352,"name":"Svjetlana Kalanj Bognar","orcid":"0000-0002-5713-4910","position":10,"is_corresponding":false},{"id":595017,"name":"Rodrigo Herrera‐Molina","orcid":"0000-0001-9597-5131","position":11,"is_corresponding":false},{"id":705353,"name":"Kristina Mlinac","orcid":"0000-0002-4309-5447","position":12,"is_corresponding":false},{"id":705345,"name":"Katarina Ilić","orcid":"0000-0002-8995-6396","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-18T23:46:28.747384Z","pmid":"34948386","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}