{"doi":"10.3390/ijms222413448","title":"Effects of Heme Oxygenase-1 on c-Kit-Positive Cardiac Cells","abstract":"Heme oxygenase-1 (HO-1) is one of the most powerful cytoprotective proteins known. The goal of this study was to explore the effects of HO-1 in c-kit-positive cardiac cells (CPCs). LinNEG/c-kitPOS CPCs were isolated and expanded from wild-type (WT), HO-1 transgenic (TG), or HO-1 knockout (KO) mouse hearts. Compared with WT CPCs, cell proliferation was significantly increased in HO-1TG CPCs and decreased in HO-1KO CPCs. HO-1TG CPCs also exhibited a marked increase in new DNA synthesis during the S-phase of cell division, not only under normoxia (21% O2) but after severe hypoxia (1% O2 for 16 h). These properties of HO-1TG CPCs were associated with nuclear translocation (and thus activation) of Nrf2, a key transcription factor that regulates antioxidant genes, and increased protein expression of Ec-SOD, the only extracellular antioxidant enzyme. These data demonstrate that HO-1 upregulates Ec-SOD in CPCs and suggest that this occurs via activation of Nrf2, which thus is potentially involved in the crosstalk between two antioxidants, HO-1 in cytoplasm and Ec-SOD in extracellular matrix. Overexpression of HO-1 in CPCs may improve the survival and reparative ability of CPCs after transplantation and thus may have potential clinical application to increase efficacy of cell therapy.","journal":"International Journal of Molecular Sciences","year":2021,"id":196397,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9651,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":768959,"name":"Chandrashekhar Dasari","orcid":"0000-0002-7526-2190","position":1,"is_corresponding":false},{"id":612115,"name":"Ding Li","orcid":"0000-0002-3581-7630","position":2,"is_corresponding":false},{"id":768960,"name":"Asma Arshia","orcid":"0000-0001-8166-288X","position":3,"is_corresponding":false},{"id":768961,"name":"Ahmed Muaaz Umer","orcid":"0009-0008-2594-8435","position":4,"is_corresponding":false},{"id":769679,"name":"Mohamed Riad Abdelgawad Abouzid","orcid":null,"position":5,"is_corresponding":false},{"id":420793,"name":"Yiru Guo","orcid":"0000-0002-0614-9899","position":6,"is_corresponding":false},{"id":353346,"name":"Roberto Bolli","orcid":"0000-0002-9160-8614","position":7,"is_corresponding":false},{"id":768958,"name":"Qianhong Li","orcid":"0000-0001-5275-3897","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-18T23:50:15.704473Z","pmid":"34948245","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}