{"doi":"10.3390/ijms22041811","title":"Nanopore Sequencing Resolves Elusive Long Tandem-Repeat Regions in Mitochondrial Genomes","abstract":"Long non-coding, tandem-repetitive regions in mitochondrial (mt) genomes of many metazoans have been notoriously difficult to characterise accurately using conventional sequencing methods. Here, we show how the use of a third-generation (long-read) sequencing and informatic approach can overcome this problem. We employed Oxford Nanopore technology to sequence genomic DNAs from a pool of adult worms of the carcinogenic parasite, Schistosoma haematobium, and used an informatic workflow to define the complete mt non-coding region(s). Using long-read data of high coverage, we defined six dominant mt genomes of 33.4 kb to 22.6 kb. Although no variation was detected in the order or lengths of the protein-coding genes, there was marked length (18.5 kb to 7.6 kb) and structural variation in the non-coding region, raising questions about the evolution and function of what might be a control region that regulates mt transcription and/or replication. The discovery here of the largest tandem-repetitive, non-coding region (18.5 kb) in a metazoan organism also raises a question about the completeness of some of the mt genomes of animals reported to date, and stimulates further explorations using a Nanopore-informatic workflow.","journal":"International Journal of Molecular Sciences","year":2021,"id":164095,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9384,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":396809,"name":"Robin B. Gasser","orcid":"0000-0002-4423-1690","position":1,"is_corresponding":false},{"id":268945,"name":"Bonnie L. Webster","orcid":"0000-0003-0930-9314","position":2,"is_corresponding":false},{"id":268464,"name":"David Rollinson","orcid":"0000-0003-1999-1716","position":3,"is_corresponding":false},{"id":684853,"name":"D. Timothy J. Littlewood","orcid":"0000-0002-2718-4001","position":4,"is_corresponding":false},{"id":684854,"name":"Bill C. H. Chang","orcid":"0000-0001-5313-4852","position":5,"is_corresponding":false},{"id":684855,"name":"Andreas J. Stroehlein","orcid":"0000-0001-9432-9816","position":6,"is_corresponding":false},{"id":396808,"name":"Pasi K. Korhonen","orcid":"0000-0002-9957-4674","position":7,"is_corresponding":false},{"id":396810,"name":"Neil D. Young","orcid":"0000-0001-8756-229X","position":8,"is_corresponding":false},{"id":684852,"name":"Liina Kinkar","orcid":"0000-0002-0511-4694","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-18T23:45:27.031465Z","pmid":"33670420","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}