{"doi":"10.3390/ijms20081929","title":"Aminoacyl-tRNA Synthetases and tRNAs for an Expanded Genetic Code: What Makes them Orthogonal?","abstract":"<jats:p>In the past two decades, tRNA molecules and their corresponding aminoacyl-tRNA synthetases (aaRS) have been extensively used in synthetic biology to genetically encode post-translationally modified and unnatural amino acids. In this review, we briefly examine one fundamental requirement for the successful application of tRNA/aaRS pairs for expanding the genetic code. This requirement is known as “orthogonality”—the ability of a tRNA and its corresponding aaRS to interact exclusively with each other and avoid cross-reactions with additional types of tRNAs and aaRSs in a given organism.</jats:p>","journal":"International Journal of Molecular Sciences","year":2019,"id":31616,"datarank":1.375349232871292,"base_score":3.713572066704308,"endowment":3.713572066704308,"self_citation_contribution":0.5570358100056463,"citation_network_contribution":0.8183134228656457,"self_endowment_contribution":0.5570358100056463,"citer_contribution":0.8183134228656457,"corpus_percentile":null,"corpus_rank":null,"citation_count":40,"citer_count":39,"citers_with_citation_signal":33,"citers_with_endowment":33,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":169060,"name":"Dieter Söll","orcid":"0000-0002-3077-8986","position":1,"is_corresponding":false},{"id":17852,"name":"Sergey V. 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