{"doi":"10.3390/genes12121852","title":"SESN3 Inhibited SMAD3 to Relieve Its Suppression for MiR-124, Thus Regulating Pre-Adipocyte Adipogenesis","abstract":"<jats:p>Sestrin-3, together with the other two members Sestrin-1 and Sestrin-2, belongs to the Sestrin family. The Sestrin protein family has been demonstrated to be involved in antioxidative, metabolic homeostasis, and even the development of nonalcoholic steatohepatitis (NASH). However, the adipogenic regulatory role of SESN3 in adipogenesis still needs to be further explored. In this study, we demonstrated SESN3 inhibited porcine pre-adipocyte proliferation, thus suppressing its adipogenesis. Meanwhile, SESN3 has been demonstrated to inhibit Smad3 thus protecting against NASH. Further, for our previous study, we found mmu-miR-124 involved in 3T3-L1 cell adipogenesis regulation. In this study, we also identified that ssc-miR-124 inhibited porcine pre-adipocyte proliferation, thus suppressing its adipogenesis, and the SMAD3 was an inhibitor of ssc-miR-124 by binding to its promoter. Furthermore, the ssc-miR-124 targeted porcine C/EBPα and GR and thus inhibited pre-adipocyte adipogenesis. In conclusion, SESN3 inhibited SMAD3, thus improving ssc-miR124, and then suppressed C/EBPα and GR to regulate pre-adipocytes adipogenesis.</jats:p>","journal":"Genes","year":2021,"id":35533,"datarank":0.562150236158966,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.2167624722098591,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.2167624722098591,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":181791,"name":"Jindi Zhao","orcid":null,"position":1,"is_corresponding":false},{"id":181792,"name":"Mengting Yan","orcid":null,"position":2,"is_corresponding":false},{"id":181793,"name":"Xuexin Li","orcid":null,"position":3,"is_corresponding":false},{"id":75361,"name":"Kai Yang","orcid":"0000-0002-9207-8061","position":4,"is_corresponding":false},{"id":109732,"name":"Wei Wei","orcid":"0000-0002-1018-7708","position":5,"is_corresponding":false},{"id":181794,"name":"Lifan Zhang","orcid":null,"position":6,"is_corresponding":false},{"id":103790,"name":"Jie Chen","orcid":"0000-0001-7680-4978","position":7,"is_corresponding":false},{"id":181790,"name":"Weimin Lin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34946801","pmcid":"PMC8701261","openalex_id":"https://openalex.org/W3217615580","authors":[],"funders":[{"funder_name":"Joint Funds of the National Natural Science Foundation of China","grant_id":"No. U20A2052","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"Nos. 31872334 and Nos. 31902132","title":null}],"total_grants":2,"fwci":1.5488,"citation_percentile":0.85156021,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2024,"count":6},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2073-4425/12/12/1852/pdf?version=1639969477","host_type":"journal"},{"url":"https://www.mdpi.com/2073-4425/12/12/1852/pdf?version=1639969477","host_type":"GOLD"},{"url":"https://www.mdpi.com/2073-4425/12/12/1852/pdf?version=1639969477","host_type":"publisher"},{"url":"https://www.mdpi.com/2073-4425/12/12/1852/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/genes12121852","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34946801","host_type":"repository"},{"url":"https://doaj.org/article/3e1a0b8f708048208ad05f8c09a460c7","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/genes12121852","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8701261","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8701261","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8701261?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Plant Virus Research Studies","Viral Infectious Diseases and Gene Expression in Insects","Lipid metabolism and biosynthesis","Medicine","Biology","Adipocytes","Adipogenesis","Animals","Cell Differentiation","Cells, Cultured","Heat-Shock Proteins","Mice","MicroRNAs","Non-alcoholic Fatty Liver Disease","Smad3 Protein","Sestrins"],"mesh_terms":["Sestrins","Animals","Cell Differentiation","Cells, Cultured","Heat-Shock Proteins","Adipocytes","MicroRNAs","Adipogenesis","Mice","Smad3 Protein","Non-alcoholic Fatty Liver Disease"],"keywords":["Adipogenesis","Adipocyte","Chemistry","Cell biology","Nonalcoholic steatohepatitis","Downregulation and upregulation","Endocrinology","Internal medicine","Biology","Biochemistry","Adipose tissue","Medicine","Gene","Fatty liver","Nonalcoholic fatty liver disease","Pig","Smad3","Sesn3","Ssc-mir-124-3p"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T04:30:17.831682Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}