{"doi":"10.3390/cells14070481","title":"Opposite Roles of IL-32α Versus IL-32β/γ Isoforms in Promoting Monocyte-Derived Osteoblast/Osteoclast Differentiation and Vascular Calcification in People with HIV","abstract":"People with HIV (PWH) have an increased risk of developing cardiovascular disease (CVD). Our recent data demonstrated that the multi-isoform proinflammatory cytokine IL-32 is upregulated in PWH and is associated with arterial stiffness and subclinical atherosclerosis. However, the mechanisms by which IL-32 contributes to the pathogenesis of these diseases remain unclear. Here, we show that while the less expressed IL-32α isoform induces the differentiation of human classical monocytes into the calcium-resorbing osteoclast cells, the dominantly expressed isoforms IL-32β and IL-32γ suppress this function through the inhibition of TGF-β and induce the differentiation of monocytes into the calcium-depositing osteocalcin+ osteoblasts. These results aligned with the increase in plasma levels of osteoprotegerin, a biomarker of vascular calcification, and its association with the presence of coronary artery subclinical atherosclerosis and calcium score in PWH. These findings support a novel role for the proinflammatory cytokine IL-32 in the pathophysiology of CVD by increasing vascular calcification in PWH.","journal":"Cells","year":2025,"id":519271,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9454,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1054587,"name":"Aurélie Cleret‐Buhot","orcid":null,"position":1,"is_corresponding":false},{"id":660819,"name":"Mohamed Sylla","orcid":null,"position":2,"is_corresponding":false},{"id":660820,"name":"Rémi Bunet","orcid":null,"position":3,"is_corresponding":false},{"id":1387608,"name":"Florent Bertrand","orcid":"0000-0003-4516-7587","position":4,"is_corresponding":false},{"id":1388189,"name":"Marc-Messier Peet","orcid":null,"position":5,"is_corresponding":false},{"id":659747,"name":"Carl Chartrand‐Lefebvre","orcid":"0000-0002-2299-7855","position":6,"is_corresponding":false},{"id":659751,"name":"Benoît Trottier","orcid":"0000-0002-9285-848X","position":7,"is_corresponding":false},{"id":659752,"name":"Réjean Thomas","orcid":"0000-0002-9112-3561","position":8,"is_corresponding":false},{"id":233493,"name":"Jean‐Pierre Routy","orcid":"0000-0001-9897-7589","position":9,"is_corresponding":false},{"id":688325,"name":"Claude Fortin","orcid":"0000-0001-9334-3093","position":10,"is_corresponding":false},{"id":623265,"name":"Valérie Martel‐Laferrière","orcid":"0000-0001-7973-8582","position":11,"is_corresponding":false},{"id":659748,"name":"Manel Sadouni","orcid":"0000-0003-4751-7675","position":12,"is_corresponding":false},{"id":688321,"name":"Guy Cloutier","orcid":"0000-0002-4957-4844","position":13,"is_corresponding":false},{"id":1387609,"name":"Louise Allard","orcid":"0000-0002-8204-9056","position":14,"is_corresponding":false},{"id":246786,"name":"Jorge R. 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