{"doi":"10.3390/cells13231992","title":"Class B Scavenger Receptor CD36 as a Potential Therapeutic Target in Inflammation Induced by Danger-Associated Molecular Patterns","abstract":"The class B scavenger receptor CD36 is known to bind and mediate the transport of lipid-related ligands and it functions as a pattern recognition receptor (PRR) for a variety of pathogens, including bacteria and viruses. In this study, we assessed CD36's role as a PRR mediating pro-inflammatory effects of several known Danger-Associated Molecular Patterns (DAMPs) used either as a single preparation or as a combination of DAMPs in the form of total cell/skeletal muscle tissue lysates. Our data demonstrated that multiple DAMPs, including HMGB1, HSPs, histone H3, SAA, and oxPAPC, as well as cell/tissue lysate preparations, induced substantially higher (~7-10-fold) IL-8 cytokine responses in HEK293 cells overexpressing CD36 compared to control WT cells. At the same time, DAMP-induced secretion of IL-6 in bone marrow-derived macrophages (BMDM) from CD36-/- mice was markedly (~2-3 times) reduced, as compared to macrophages from normal mice. Synthetic amphipathic helical peptides (SAHPs), known CD36 ligands, efficiently blocked CD36-dependent inflammatory responses induced by both cell and tissue lysates, HMGB1 and histone H3 in CD36+ cells. IP injection of total cellular lysate preparation induced inflammatory responses that were assessed by the expression of liver and lung pro-inflammatory markers, including IL-6, TNF-α, CD68, and CXCL1, and was reduced by ~50% in CD36-deficient mice compared to normal mice. Our findings demonstrate that CD36 is a PRR contributing to the innate immune response via mediating DAMP-induced inflammatory signaling and highlight the importance of this receptor as a potential therapeutic target in DAMP-associated inflammatory conditions.","journal":"Cells","year":2024,"id":439387,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9658,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":534771,"name":"Alexander V. Bocharov","orcid":"0000-0002-3482-2980","position":1,"is_corresponding":false},{"id":535622,"name":"Tatyana G. Vishnyakova","orcid":null,"position":2,"is_corresponding":false},{"id":534773,"name":"Zhigang Chen","orcid":"0000-0002-0797-7215","position":3,"is_corresponding":false},{"id":414575,"name":"Yunbo Ke","orcid":"0000-0002-4151-9058","position":4,"is_corresponding":false},{"id":414581,"name":"Anna A. Birukova","orcid":"0000-0003-0535-7693","position":5,"is_corresponding":false},{"id":289010,"name":"Peter S.T. Yuen","orcid":"0000-0001-9557-3909","position":6,"is_corresponding":false},{"id":627890,"name":"Takayuki Tsuji","orcid":"0000-0003-3120-5562","position":7,"is_corresponding":false},{"id":461361,"name":"Robert A. Star","orcid":"0000-0003-0940-3079","position":8,"is_corresponding":false},{"id":414580,"name":"Konstantin G. Birukov","orcid":"0000-0002-0303-5394","position":9,"is_corresponding":false},{"id":535625,"name":"Amy P. Patterson","orcid":null,"position":10,"is_corresponding":false},{"id":535624,"name":"Thomas L. Eggerman","orcid":null,"position":11,"is_corresponding":false},{"id":534772,"name":"Irina N. Baranova","orcid":"0000-0003-4250-1340","position":0,"is_corresponding":true}],"reference_count":76,"raw_metadata":null,"created_at":"2026-07-19T02:00:47.992543Z","pmid":"39682740","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}