{"doi":"10.3390/cells13020126","title":"Coronavirus M Protein Trafficking in Epithelial Cells Utilizes a Myosin Vb Splice Variant and Rab10","abstract":"The membrane (M) glycoprotein of coronaviruses (CoVs) serves as the nidus for virion assembly. Using a yeast two-hybrid screen, we identified the interaction of the cytosolic tail of Murine Hepatitis Virus (MHV-CoV) M protein with Myosin Vb (MYO5B), specifically with the alternative splice variant of cellular MYO5B including exon D (MYO5B+D), which mediates interaction with Rab10. When co-expressed in human lung epithelial A549 and canine kidney epithelial MDCK cells, MYO5B+D co-localized with the MHV-CoV M protein, as well as with the M proteins from Porcine Epidemic Diarrhea Virus (PEDV-CoV), Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). Co-expressed M proteins and MYO5B+D co-localized with endogenous Rab10 and Rab11a. We identified point mutations in MHV-CoV M that blocked the interaction with MYO5B+D in yeast 2-hybrid assays. One of these point mutations (E121K) was previously shown to block MHV-CoV virion assembly and its interaction with MYO5B+D. The E to K mutation at homologous positions in PEDV-CoV, MERS-CoV and SARS-CoV-2 M proteins also blocked colocalization with MYO5B+D. The knockdown of Rab10 blocked the co-localization of M proteins with MYO5B+D and was rescued by re-expression of CFP-Rab10. Our results suggest that CoV M proteins traffic through Rab10-containing systems, in association with MYO5B+D.","journal":"Cells","year":2024,"id":464560,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9626,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":86856,"name":"Joseph T. Roland","orcid":"0000-0001-9977-0520","position":1,"is_corresponding":false},{"id":1295861,"name":"Elizabeth H. Manning","orcid":null,"position":2,"is_corresponding":false},{"id":1104682,"name":"Catherine Caldwell","orcid":null,"position":3,"is_corresponding":false},{"id":1168548,"name":"Honor L. Glenn","orcid":"0000-0002-8148-4224","position":4,"is_corresponding":false},{"id":637926,"name":"Pierre‐Olivier Vidalain","orcid":"0000-0003-3082-7407","position":5,"is_corresponding":false},{"id":769295,"name":"Frédéric Tangy","orcid":"0000-0002-5937-5654","position":6,"is_corresponding":false},{"id":260546,"name":"Brenda G. Hogue","orcid":"0000-0002-4545-9208","position":7,"is_corresponding":false},{"id":109651,"name":"Cornelis A. M. de Haan","orcid":"0000-0002-4459-9874","position":8,"is_corresponding":false},{"id":86851,"name":"James R. Goldenring","orcid":"0000-0002-7833-2940","position":9,"is_corresponding":false},{"id":879554,"name":"Lynne A. Lapierre","orcid":"0000-0002-7163-0830","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T02:04:42.082401Z","pmid":"38247817","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}