{"doi":"10.3390/cells12212582","title":"DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation","abstract":"Spermatogonial stem cell (SSC) transplantation into the testis of a germ cell (GC)-depleted surrogate allows transmission of donor genotype via donor-derived sperm produced by the recipient. Transplantation of gene-edited SSCs provides an approach to propagate gene-edited large animal models. DAZL is a conserved RNA-binding protein important for GC development, and DAZL knockout (KO) causes defects in GC commitment and differentiation. We characterized DAZL-KO pigs as SSC transplantation recipients. While there were GCs in 1-week-old (wko) KO, complete GC depletion was observed by 10 wko. Donor GCs were transplanted into 18 DAZL-KO recipients at 10–13 wko. At sexual maturity, semen and testes were evaluated for transplantation efficiency and spermatogenesis. Approximately 22% of recipient seminiferous tubules contained GCs, including elongated spermatids and proliferating spermatogonia. The ejaculate of 89% of recipients contained sperm, exclusively from donor origin. However, sperm concentration was lower than the wild-type range. Testicular protein expression and serum hormonal levels were comparable between DAZL-KO and wild-type. Intratesticular testosterone and Leydig cell volume were increased, and Leydig cell number decreased in transplanted DAZL-KO testis compared to wild-type. In summary, DAZL-KO pigs support donor-derived spermatogenesis following SSC transplantation, but low spermatogenic efficiency currently limits their use for the production of offspring.","journal":"Cells","year":2023,"id":367577,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9555,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":364702,"name":"Taylor Goldsmith","orcid":"0009-0002-4247-9884","position":1,"is_corresponding":false},{"id":688395,"name":"P. Rodríguez-Villamil","orcid":"0009-0004-6982-7791","position":2,"is_corresponding":false},{"id":467473,"name":"Felipe Ledur Ongaratto","orcid":"0000-0002-3052-8768","position":3,"is_corresponding":false},{"id":784467,"name":"Staci L. Solin","orcid":null,"position":4,"is_corresponding":false},{"id":256878,"name":"Dennis A. Webster","orcid":null,"position":5,"is_corresponding":false},{"id":638950,"name":"Uyanga Ganbaatar","orcid":"0000-0002-1561-6516","position":6,"is_corresponding":false},{"id":1054314,"name":"Shane Hodgson","orcid":"0000-0002-1552-9359","position":7,"is_corresponding":false},{"id":832213,"name":"Stanislas Corbiere","orcid":"0000-0001-9329-712X","position":8,"is_corresponding":false},{"id":784466,"name":"Alla Bondareva","orcid":null,"position":9,"is_corresponding":false},{"id":254330,"name":"Daniel F. Carlson","orcid":"0000-0001-6095-2108","position":10,"is_corresponding":false},{"id":364704,"name":"Ina Dobrinski","orcid":"0000-0002-0695-2606","position":11,"is_corresponding":false},{"id":710346,"name":"Nathália de Lima e Martins Lara","orcid":"0000-0002-8170-6384","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T01:15:16.306414Z","pmid":"37947660","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}