{"doi":"10.3390/cells12050760","title":"Human Bone Marrow-Derived Mesenchymal Stromal Cells Reduce the Severity of Experimental Necrotizing Enterocolitis in a Concentration-Dependent Manner","abstract":"Necrotizing enterocolitis (NEC) is a devastating gut disease in preterm neonates. In NEC animal models, mesenchymal stromal cells (MSCs) administration has reduced the incidence and severity of NEC. We developed and characterized a novel mouse model of NEC to evaluate the effect of human bone marrow-derived MSCs (hBM-MSCs) in tissue regeneration and epithelial gut repair. NEC was induced in C57BL/6 mouse pups at postnatal days (PND) 3–6 by (A) gavage feeding term infant formula, (B) hypoxia/hypothermia, and (C) lipopolysaccharide. Intraperitoneal injections of PBS or two hBM-MSCs doses (0.5 × 106 or 1 × 106) were given on PND2. At PND 6, we harvested intestine samples from all groups. The NEC group showed an incidence of NEC of 50% compared with controls (p < 0.001). Severity of bowel damage was reduced by hBM-MSCs compared to the PBS-treated NEC group in a concentration-dependent manner, with hBM-MSCs (1 × 106) inducing a NEC incidence reduction of up to 0% (p < 0.001). We showed that hBM-MSCs enhanced intestinal cell survival, preserving intestinal barrier integrity and decreasing mucosal inflammation and apoptosis. In conclusion, we established a novel NEC animal model and demonstrated that hBM-MSCs administration reduced the NEC incidence and severity in a concentration-dependent manner, enhancing intestinal barrier integrity.","journal":"Cells","year":2023,"id":361795,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1114549,"name":"Andrea Tomaselli","orcid":"0000-0003-2419-2716","position":1,"is_corresponding":false},{"id":1114550,"name":"Genny Raffaeli","orcid":"0000-0001-9175-9394","position":2,"is_corresponding":false},{"id":731213,"name":"Stefania Crippa","orcid":"0000-0003-0051-4872","position":3,"is_corresponding":false},{"id":1114551,"name":"Cristina Arribas","orcid":"0000-0002-7916-4571","position":4,"is_corresponding":false},{"id":1114552,"name":"Ilaria Amodeo","orcid":"0000-0002-2650-0084","position":5,"is_corresponding":false},{"id":1114553,"name":"Silvia Gulden","orcid":"0000-0002-4309-6626","position":6,"is_corresponding":false},{"id":1114554,"name":"Giacomo Simeone Amelio","orcid":"0000-0002-2519-3043","position":7,"is_corresponding":false},{"id":1114555,"name":"Valeria Cortesi","orcid":"0000-0003-2083-411X","position":8,"is_corresponding":false},{"id":432650,"name":"Francesca Manzoni","orcid":"0000-0002-3656-0910","position":9,"is_corresponding":false},{"id":1114556,"name":"Gaia Cervellini","orcid":"0000-0003-0992-7841","position":10,"is_corresponding":false},{"id":1114557,"name":"Jacopo Cerasani","orcid":"0000-0002-2474-0789","position":11,"is_corresponding":false},{"id":1114986,"name":"Camilla Menis","orcid":null,"position":12,"is_corresponding":false},{"id":1114558,"name":"Nicola Pesenti","orcid":"0000-0002-2475-7927","position":13,"is_corresponding":false},{"id":1114987,"name":"Matteo Tripodi","orcid":null,"position":14,"is_corresponding":false},{"id":1114559,"name":"Ludovica Santi","orcid":"0000-0001-5425-213X","position":15,"is_corresponding":false},{"id":1114560,"name":"Marco Maggioni","orcid":"0000-0001-8603-8998","position":16,"is_corresponding":false},{"id":872989,"name":"Caterina Lonati","orcid":"0000-0001-7855-7851","position":17,"is_corresponding":false},{"id":1114988,"name":"Samanta Oldoni","orcid":null,"position":18,"is_corresponding":false},{"id":1114561,"name":"Francesca Algieri","orcid":"0000-0002-0262-4867","position":19,"is_corresponding":false},{"id":1114562,"name":"Felipe Garrido","orcid":"0000-0002-8835-0667","position":20,"is_corresponding":false},{"id":1114563,"name":"Maria Ester Bernardo","orcid":"0000-0002-0099-8021","position":21,"is_corresponding":false},{"id":1114564,"name":"Fabio Mosca","orcid":"0000-0001-6477-0299","position":22,"is_corresponding":false},{"id":1114565,"name":"Giacomo Cavallaro","orcid":"0000-0002-4921-1437","position":23,"is_corresponding":false},{"id":1114548,"name":"Livia Provitera","orcid":"0000-0002-0114-6541","position":0,"is_corresponding":true}],"reference_count":104,"raw_metadata":null,"created_at":"2026-07-19T01:14:15.063476Z","pmid":"36899900","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}