{"doi":"10.3390/cells11233883","title":"Autophagy Induced by Toll-like Receptor Ligands Regulates Antigen Extraction and Presentation by B Cells","abstract":"The engagement of B cells with surface-tethered antigens triggers the formation of an immune synapse (IS), where the local secretion of lysosomes can facilitate antigen uptake. Lysosomes intersect with other intracellular processes, such as Toll-like Receptor (TLR) signaling and autophagy coordinating immune responses. However, the crosstalk between these processes and antigen presentation remains unclear. Here, we show that TLR stimulation induces autophagy in B cells and decreases their capacity to extract and present immobilized antigens. We reveal that TLR stimulation restricts lysosome repositioning to the IS by triggering autophagy-dependent degradation of GEF-H1, a Rho GTP exchange factor required for stable lysosome recruitment at the synaptic membrane. GEF-H1 degradation is not observed in B cells that lack αV integrins and are deficient in TLR-induced autophagy. Accordingly, these cells show efficient antigen extraction in the presence of TLR stimulation, confirming the role of TLR-induced autophagy in limiting antigen extraction. Overall, our results suggest that resources associated with autophagy regulate TLR and BCR-dependent functions, which can finetune antigen uptake by B cells. This work helps to understand the mechanisms by which B cells are activated by surface-tethered antigens in contexts of subjacent inflammation before antigen recognition, such as sepsis.","journal":"Cells","year":2022,"id":277298,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.952,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":730019,"name":"Sara Sagadiev","orcid":"0000-0002-8371-0977","position":1,"is_corresponding":false},{"id":948380,"name":"Jheimmy Diaz","orcid":"0000-0001-7772-7636","position":2,"is_corresponding":false},{"id":948381,"name":"Juan Pablo Bozo","orcid":"0009-0006-5936-1531","position":3,"is_corresponding":false},{"id":948382,"name":"Fanny Guzmán","orcid":"0000-0002-7829-0568","position":4,"is_corresponding":false},{"id":948383,"name":"Caroline Stefani","orcid":"0000-0003-3420-432X","position":5,"is_corresponding":false},{"id":948384,"name":"Silvana Zanlungo","orcid":"0000-0001-8383-9829","position":6,"is_corresponding":false},{"id":730023,"name":"Mridu Acharya","orcid":"0000-0001-7078-2560","position":7,"is_corresponding":false},{"id":948385,"name":"María-Isabel Yuseff","orcid":"0000-0001-8172-3785","position":8,"is_corresponding":false},{"id":948379,"name":"Jonathan Lagos","orcid":"0000-0003-4270-6813","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-19T00:28:34.768552Z","pmid":"36497137","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}