{"doi":"10.3390/cells11121965","title":"Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma","abstract":"Exosomes that are released by T cells are key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires their isolation from a very heterogeneous mixture of extracellular vesicles that are present in the human plasma. It has been shown that exosomes that are produced by T cells could be isolated from plasma by immune capture using antibodies that target the CD3 antigen, which is a key component of the TCR complex that is present in all T lymphocytes. Here, we demonstrate that CD3(+) exosomes that are isolated from plasma can be used for high-throughput molecular profiling using proteomics and metabolomics tools. This profiling allowed for the identification of proteins and metabolites that differentiated the CD3(+) from the CD3(-) exosome fractions that were present in the plasma of healthy donors. Importantly, the proteins and metabolites that accumulated in the CD3(+) vesicles reflected the known molecular features of T lymphocytes. Hence, CD3(+) exosomes that are isolated from human plasma by immune capture could serve as a \"T cell biopsy\".","journal":"Cells","year":2022,"id":255447,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9492,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":903656,"name":"Karol Jelonek","orcid":"0000-0002-7233-7695","position":1,"is_corresponding":false},{"id":326161,"name":"Sujan Kumar Mondal","orcid":"0000-0003-3241-2504","position":2,"is_corresponding":false},{"id":903657,"name":"Marta Gawin","orcid":"0000-0002-2834-5129","position":3,"is_corresponding":false},{"id":903658,"name":"Katarzyna Mrowiec","orcid":"0000-0001-6181-9122","position":4,"is_corresponding":false},{"id":864481,"name":"Piotr Wiland","orcid":"0000-0001-9999-0267","position":5,"is_corresponding":false},{"id":225451,"name":"Theresa L. Whiteside","orcid":"0000-0001-7316-6181","position":6,"is_corresponding":false},{"id":864480,"name":"Monika Pietrowska","orcid":"0000-0001-9317-7822","position":7,"is_corresponding":false},{"id":903655,"name":"Aneta Zebrowska","orcid":"0000-0001-6049-301X","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-19T00:25:17.224217Z","pmid":"35741093","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}