{"doi":"10.3390/cells11020256","title":"Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C","abstract":"Aberrant remodeling of the asthmatic airway is not well understood but is thought to be attributable in part to mechanical compression of airway epithelial cells. Here, we examine compression-induced expression and secretion of the extracellular matrix protein tenascin C (TNC) from well-differentiated primary human bronchial epithelial (HBE) cells grown in an air–liquid interface culture. We measured TNC mRNA expression using RT-qPCR and secreted TNC protein using Western blotting and ELISA. To determine intracellular signaling pathways, we used specific inhibitors for either ERK or TGF-β receptor, and to assess the release of extracellular vesicles (EVs) we used a commercially available kit and Western blotting. At baseline, secreted TNC protein was significantly higher in asthmatic compared to non-asthmatic cells. In response to mechanical compression, both TNC mRNA expression and secreted TNC protein was significantly increased in both non-asthmatic and asthmatic cells. TNC production depended on both the ERK and TGF-β receptor pathways. Moreover, mechanically compressed HBE cells released EVs that contain TNC. These data reveal a novel mechanism by which mechanical compression, as is caused by bronchospasm, is sufficient to induce the production of ECM protein in the airway and potentially contribute to airway remodeling.","journal":"Cells","year":2022,"id":244342,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":29,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.954,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":585790,"name":"Thien‐Khoi N. Phung","orcid":"0000-0003-1831-3171","position":1,"is_corresponding":false},{"id":247347,"name":"Michael J. O’Sullivan","orcid":"0000-0003-0873-0350","position":2,"is_corresponding":false},{"id":247345,"name":"Jennifer A. Mitchel","orcid":"0000-0002-4480-3193","position":3,"is_corresponding":false},{"id":320640,"name":"Margherita De Marzio","orcid":"0000-0003-1821-208X","position":4,"is_corresponding":false},{"id":320637,"name":"Ayşe Kılıç","orcid":"0000-0003-3217-4075","position":5,"is_corresponding":false},{"id":25033,"name":"Scott T. Weiss","orcid":"0000-0001-7196-303X","position":6,"is_corresponding":false},{"id":247351,"name":"Jeffrey J. Fredberg","orcid":"0000-0002-7968-0920","position":7,"is_corresponding":false},{"id":247354,"name":"Jin‐Ah Park","orcid":"0000-0002-8930-9287","position":8,"is_corresponding":false},{"id":689895,"name":"Chimwemwe Mwase","orcid":"0000-0002-0746-2799","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T00:23:25.718788Z","pmid":"35053372","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}