{"doi":"10.3390/cancers14102427","title":"Current and Emerging Approaches to Study Microenvironmental Interactions and Drug Activity in Classical Hodgkin Lymphoma","abstract":"<jats:p>Classic Hodgkin lymphoma is characterized by a few tumor cells surrounded by a protective and immunosuppressive tumor microenvironment (TME) composed by a wide variety of noncancerous cells that are an active part of the disease. Therefore, new techniques to study the cHL-TME and new therapeutic strategies targeting specifically tumor cells, reactivating the antitumor immunity, counteracting the protective effects of the TME, were developed. Here, we describe new methods used to study the cell composition, the phenotype, and the spatial distribution of Hodgkin and Reed–Sternberg (HRS) cells and of noncancerous cells in tumor tissues. Moreover, we propose a classification, with increasing complexity, of the in vitro functional studies used to clarify the interactions leading not only to HRS cell survival, growth and drug resistance, but also to the immunosuppressive tumor education of monocytes, T lymphocytes and fibroblasts. This classification also includes new 3-dimensional (3D) models, obtained by cultivating HRS cells in extracellular matrix scaffolds or in sponge scaffolds, under non-adherent conditions with noncancerous cells to form heterospheroids (HS), implanted in developing chick eggs (ovo model). We report results obtained with these approaches and their applications in clinical setting.</jats:p>","journal":"Cancers","year":2022,"id":637771,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1656151,"name":"Cinzia Borghese","orcid":null,"position":1,"is_corresponding":false},{"id":1656153,"name":"Donatella Aldinucci","orcid":"0000-0002-4548-3566","position":2,"is_corresponding":false},{"id":1656150,"name":"Naike Casagrande","orcid":"0000-0001-5352-5677","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Current and Emerging Approaches to Study Microenvironmental Interactions and Drug Activity in Classical Hodgkin Lymphoma","abstract":"<jats:p>Classic Hodgkin lymphoma is characterized by a few tumor cells surrounded by a protective and immunosuppressive tumor microenvironment (TME) composed by a wide variety of noncancerous cells that are an active part of the disease. Therefore, new techniques to study the cHL-TME and new therapeutic strategies targeting specifically tumor cells, reactivating the antitumor immunity, counteracting the protective effects of the TME, were developed. Here, we describe new methods used to study the cell composition, the phenotype, and the spatial distribution of Hodgkin and Reed–Sternberg (HRS) cells and of noncancerous cells in tumor tissues. Moreover, we propose a classification, with increasing complexity, of the in vitro functional studies used to clarify the interactions leading not only to HRS cell survival, growth and drug resistance, but also to the immunosuppressive tumor education of monocytes, T lymphocytes and fibroblasts. This classification also includes new 3-dimensional (3D) models, obtained by cultivating HRS cells in extracellular matrix scaffolds or in sponge scaffolds, under non-adherent conditions with noncancerous cells to form heterospheroids (HS), implanted in developing chick eggs (ovo model). We report results obtained with these approaches and their applications in clinical setting.</jats:p>","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35626032","pmcid":"PMC9139207","openalex_id":"https://openalex.org/W4280580346","authors":[],"funders":[{"funder_name":"Italian Association for Cancer Research","grant_id":"15844","title":null},{"funder_name":"Italian Association for Cancer Research","grant_id":"IG 15844","title":null},{"funder_name":"Italian Ministry of Health (Ricerca Corrente)","grant_id":"NA","title":null}],"total_grants":3,"fwci":0.1508,"citation_percentile":0.38639734,"influential_citations":0,"citation_trend":[{"year":2023,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2072-6694/14/10/2427/pdf?version=1652510474","host_type":"journal"},{"url":"https://www.mdpi.com/2072-6694/14/10/2427/pdf?version=1652510474","host_type":"publisher"},{"url":"https://www.mdpi.com/2072-6694/14/10/2427/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/cancers14102427","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35626032","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/cancers14102427","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9139207","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9139207","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9139207?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Immune cells in cancer","Immunotherapy and Immune Responses","Cancer Cells and Metastasis"],"mesh_terms":[],"keywords":["Tumor microenvironment","Extracellular matrix","Lymphoma","Cancer research","In vitro","Phenotype","Drug resistance","Biology","Tumor cells","Immunology","Cell biology","Classical Hodgkin lymphoma","immunosuppression","Heterospheroids","Multiplex Immunohistochemistry","Drug Response Testing","Stroma-mediated Drug Resistance","Tumor Education"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T19:29:03.265183Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}