{"doi":"10.3390/cancers12092583","title":"Temozolomide-Induced RNA Interactome Uncovers Novel LncRNA Regulatory Loops in Glioblastoma","abstract":"Resistance to chemotherapy by temozolomide (TMZ) is a major cause of glioblastoma (GBM) recurrence. So far, attempts to characterize factors that contribute to TMZ sensitivity have largely focused on protein-coding genes, and failed to provide effective therapeutic targets. Long noncoding RNAs (lncRNAs) are essential regulators of epigenetic-driven cell diversification, yet, their contribution to the transcriptional response to drugs is less understood. Here, we performed RNA-seq and small RNA-seq to provide a comprehensive map of transcriptome regulation upon TMZ in patient-derived GBM stem-like cells displaying different drug sensitivity. In a search for regulatory mechanisms, we integrated thousands of molecular associations stored in public databases to generate a background \"RNA interactome\". Our systems-level analysis uncovered a coordinated program of TMZ response reflected by regulatory circuits that involve transcription factors, mRNAs, miRNAs, and lncRNAs. We discovered 22 lncRNAs involved in regulatory loops and/or with functional relevance in drug response and prognostic value in gliomas. Thus, the investigation of TMZ-induced gene networks highlights novel RNA-based predictors of chemosensitivity in GBM. The computational modeling used to identify regulatory circuits underlying drug response and prioritizing gene candidates for functional validation is applicable to other datasets.","journal":"Cancers","year":2020,"id":84211,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9477,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":432292,"name":"Arnaud Muller","orcid":"0000-0002-3841-4722","position":1,"is_corresponding":false},{"id":432293,"name":"Wei Jiang","orcid":"0000-0002-2836-7694","position":2,"is_corresponding":false},{"id":280679,"name":"Ramkrishna Mitra","orcid":"0000-0002-6181-9066","position":3,"is_corresponding":false},{"id":433225,"name":"Mohamad Sarmini","orcid":null,"position":4,"is_corresponding":false},{"id":432294,"name":"Monika Dieterle","orcid":"0000-0002-9918-6022","position":5,"is_corresponding":false},{"id":432295,"name":"Anna Golebiewska","orcid":"0000-0002-4160-2521","position":6,"is_corresponding":false},{"id":432296,"name":"Tao Ye","orcid":"0000-0002-3394-2083","position":7,"is_corresponding":false},{"id":201780,"name":"Eric Van Dyck","orcid":"0000-0001-8614-2402","position":8,"is_corresponding":false},{"id":70252,"name":"Christel Herold‐Mende","orcid":"0000-0002-1915-745X","position":9,"is_corresponding":false},{"id":13182,"name":"Zhongming Zhao","orcid":"0000-0002-3477-0914","position":10,"is_corresponding":false},{"id":432297,"name":"Francisco Azuaje","orcid":"0000-0002-9207-9072","position":11,"is_corresponding":false},{"id":432298,"name":"Simone P. Niclou","orcid":"0000-0002-3417-9534","position":12,"is_corresponding":false},{"id":432291,"name":"Sabrina Fritah","orcid":"0000-0002-7613-1424","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-18T21:54:50.827092Z","pmid":"32927769","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}