{"doi":"10.3390/cancers11081117","title":"Kinome-Wide Screening with Small Interfering RNA Identified Polo-like Kinase 1 as a Key Regulator of Proliferation in Oral Cancer Cells","abstract":"<jats:p>Oral squamous cell carcinoma (OSCC) is one of the major leading causes of cancer-related death worldwide, with limited effective markers for diagnosis and therapy, which has caused a low overall survival rate in the past decades. Kinases play important roles in tumor development and malignancy in various types of cancer. However, little is known about the role of kinases in OSCC cells. In this study, an arrayed kinome small interfering RNA (siRNA) library was used to screen oral cancer cell lines and counter assayed with normal fibroblast cells to identify the genes required for cancer cell proliferation. We found that polo-like kinase 1 (PLK1) was one of the most potent genes required for OSCC cell proliferation. The knockdown of PLK1 with a siRNA or antisense oligonucleotide (ASO) consistently diminished cyclin-B1 (CCNB1) expression/phosphorylation and the G2-M phase transition. Similar effects were observed in cells treated with the PLK1 kinase inhibitor BI6727. Besides, The Cancer Genome Atlas (TCGA) analysis revealed that PLK1 was elevated in tumor tissues and associated with short survival in patients with OSCC. We also found that PLK1 expression was highly correlated with the expression of its downstream effector, CCNB1, in patients with OSCC. Coexpression of the two genes resulted in a poor prognosis of OSCC patients, particularly those in the advanced stages of OSCC. Taken together, our results suggest that PLK1 might be a diagnostic or therapeutic marker for OSCC.</jats:p>","journal":"Cancers","year":2019,"id":676788,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":844052,"name":"Pei‐Feng Liu","orcid":"0000-0002-7849-8940","position":1,"is_corresponding":false},{"id":115772,"name":"Hsueh-Wei Chang","orcid":"0000-0003-0068-2366","position":2,"is_corresponding":false},{"id":1024480,"name":"Hung-Chih Chen","orcid":"0000-0002-2579-9723","position":3,"is_corresponding":false},{"id":1768308,"name":"Wen-Chi Chen","orcid":null,"position":4,"is_corresponding":false},{"id":1768309,"name":"Shyh-Ming Kuo","orcid":null,"position":5,"is_corresponding":false},{"id":1768310,"name":"Cheng-Hsin Lee","orcid":null,"position":6,"is_corresponding":false},{"id":844055,"name":"Chih‐Wen Shu","orcid":"0000-0002-7774-0002","position":7,"is_corresponding":false},{"id":1768307,"name":"Yih-Gang Goan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Kinome-Wide Screening with Small Interfering RNA Identified Polo-like Kinase 1 as a Key Regulator of Proliferation in Oral Cancer Cells","abstract":"<jats:p>Oral squamous cell carcinoma (OSCC) is one of the major leading causes of cancer-related death worldwide, with limited effective markers for diagnosis and therapy, which has caused a low overall survival rate in the past decades. Kinases play important roles in tumor development and malignancy in various types of cancer. However, little is known about the role of kinases in OSCC cells. In this study, an arrayed kinome small interfering RNA (siRNA) library was used to screen oral cancer cell lines and counter assayed with normal fibroblast cells to identify the genes required for cancer cell proliferation. We found that polo-like kinase 1 (PLK1) was one of the most potent genes required for OSCC cell proliferation. The knockdown of PLK1 with a siRNA or antisense oligonucleotide (ASO) consistently diminished cyclin-B1 (CCNB1) expression/phosphorylation and the G2-M phase transition. Similar effects were observed in cells treated with the PLK1 kinase inhibitor BI6727. Besides, The Cancer Genome Atlas (TCGA) analysis revealed that PLK1 was elevated in tumor tissues and associated with short survival in patients with OSCC. We also found that PLK1 expression was highly correlated with the expression of its downstream effector, CCNB1, in patients with OSCC. Coexpression of the two genes resulted in a poor prognosis of OSCC patients, particularly those in the advanced stages of OSCC. Taken together, our results suggest that PLK1 might be a diagnostic or therapeutic marker for OSCC.</jats:p>","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31387297","pmcid":"PMC6721596","openalex_id":"https://openalex.org/W2965407086","authors":[],"funders":[{"funder_name":"Ministry of Science and Technology","grant_id":"107-2311-B-214-003 and 108-2320-B-214-005-MY3","title":null},{"funder_name":"Kaohsiung Veterans General Hospital, the Kaohsiung Medical University Research Center and the Health and welfare surcharge of tobacco products, the Ministry of Health and Welfare","grant_id":"KSC107-028 KMU-TC108A04 MOHW 108-TDU-B-212-124016","title":null}],"total_grants":2,"fwci":0.9829,"citation_percentile":0.77333811,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":7},{"year":2022,"count":1},{"year":2023,"count":4},{"year":2024,"count":1},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2072-6694/11/8/1117/pdf?version=1566385011","host_type":"journal"},{"url":"https://www.mdpi.com/2072-6694/11/8/1117/pdf?version=1566385011","host_type":"publisher"},{"url":"https://www.mdpi.com/2072-6694/11/8/1117/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/cancers11081117","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31387297","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6721596","host_type":"repository"},{"url":"https://doaj.org/article/d4b74d9879404666a34e8d047a50bc6e","host_type":"repository"},{"url":"http://dx.doi.org/10.3390/cancers11081117","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6721596","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6721596?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Cancer-related Molecular Pathways","Cancer Genomics and Diagnostics","Microtubule and mitosis dynamics"],"mesh_terms":[],"keywords":["Kinome","PLK1","Cancer research","Biology","Small interfering RNA","Gene knockdown","Kinase","Polo-like kinase","Cancer","Gene silencing","Cell growth","Cancer cell","RNA interference","Cell cycle","Gene","RNA","Cell biology","Genetics","Prognosis","Biomarker","Oral Squamous Cell Carcinoma","Sirna Screening"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T03:06:28.689615Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}