{"doi":"10.3390/biom16020203","title":"Nuclear Dynamics in Quiescent Cells: Conserved Mechanisms from Yeasts to Mammals","abstract":"<jats:p>Quiescence is a reversible, non-proliferative cellular state that enables survival under nutrient limitation while preserving the capacity to resume growth. Rather than representing a passive default, quiescence is an actively regulated program conserved from unicellular eukaryotes to metazoans. This review focuses on the nuclear mechanisms underlying quiescence entry, maintenance, and exit, with primary emphasis on mechanistic insights from yeast models while highlighting conserved principles in multicellular systems. Across species, quiescence is characterized by global transcriptional repression, chromatin compaction, and the extensive reorganization of nuclear architecture, coordinated by nutrient-sensing pathways centered on TOR/mTOR signaling. We discuss how transcriptional reprogramming is achieved through redistribution of RNA polymerases, dynamic transcription factor activities, and large-scale remodeling of histone modifications, alongside repressive chromatin formation. In parallel, post-transcriptional mechanisms—including intron retention, alternative polyadenylation, and accumulation of non-coding RNAs—fine-tune gene expression while limiting biosynthetic output. We further examine how changes in nuclear organization, such as nucleolar condensation, condensin-mediated chromosome rearrangements, and telomere hyperclusters, support long-term viability and genome stability. Collectively, this review highlights nuclear dynamics as an integrative regulatory layer that links metabolic state to cellular identity, adaptability, and long-term survival, with broad implications for development, stem cell function, and disease.</jats:p>","journal":"Biomolecules","year":2026,"id":645169,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1679767,"name":"Cornelia Kilchert","orcid":"0000-0001-6834-0704","position":1,"is_corresponding":false},{"id":709154,"name":"Aydan Bulut-Karslıoğlu","orcid":"0000-0002-0413-9718","position":2,"is_corresponding":false},{"id":1679769,"name":"Myriam Ruault","orcid":"0000-0003-2990-4794","position":3,"is_corresponding":false},{"id":1331776,"name":"Angela Taddei","orcid":"0000-0002-3217-0739","position":4,"is_corresponding":false},{"id":1679770,"name":"Fatemeh Rabbani","orcid":"0009-0006-3564-6807","position":5,"is_corresponding":false},{"id":1679771,"name":"Dominika Włoch-Salamon","orcid":"0000-0002-0040-1838","position":6,"is_corresponding":false},{"id":570295,"name":"Sigurd Braun","orcid":"0000-0001-6399-8574","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Nuclear Dynamics in Quiescent Cells: Conserved Mechanisms from Yeasts to Mammals","abstract":"<jats:p>Quiescence is a reversible, non-proliferative cellular state that enables survival under nutrient limitation while preserving the capacity to resume growth. Rather than representing a passive default, quiescence is an actively regulated program conserved from unicellular eukaryotes to metazoans. This review focuses on the nuclear mechanisms underlying quiescence entry, maintenance, and exit, with primary emphasis on mechanistic insights from yeast models while highlighting conserved principles in multicellular systems. Across species, quiescence is characterized by global transcriptional repression, chromatin compaction, and the extensive reorganization of nuclear architecture, coordinated by nutrient-sensing pathways centered on TOR/mTOR signaling. We discuss how transcriptional reprogramming is achieved through redistribution of RNA polymerases, dynamic transcription factor activities, and large-scale remodeling of histone modifications, alongside repressive chromatin formation. In parallel, post-transcriptional mechanisms—including intron retention, alternative polyadenylation, and accumulation of non-coding RNAs—fine-tune gene expression while limiting biosynthetic output. We further examine how changes in nuclear organization, such as nucleolar condensation, condensin-mediated chromosome rearrangements, and telomere hyperclusters, support long-term viability and genome stability. Collectively, this review highlights nuclear dynamics as an integrative regulatory layer that links metabolic state to cellular identity, adaptability, and long-term survival, with broad implications for development, stem cell function, and disease.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41750274","pmcid":"PMC12937744","openalex_id":"https://openalex.org/W7125915898","authors":[],"funders":[{"funder_name":"Agence Nationale pour la Recherche DeSynLE","grant_id":"ANR-22-CE120013-01","title":null},{"funder_name":"European Research Council","grant_id":"101117421","title":null},{"funder_name":"German Research Foundation","grant_id":"464293512","title":null},{"funder_name":"National Science Center, Poland","grant_id":"2024/53/B/NZ8/02259","title":null},{"funder_name":"European Research Council","grant_id":"101117421, 'DOR CODE'","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Max Planck Society","grant_id":"","title":null}],"total_grants":7,"fwci":13.335,"citation_percentile":0.97784673,"influential_citations":0,"citation_trend":[{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2218-273X/16/2/203/pdf?version=1769594154","host_type":"journal"},{"url":"https://www.mdpi.com/2218-273X/16/2/203/pdf?version=1769594154","host_type":"publisher"},{"url":"https://www.mdpi.com/2218-273X/16/2/203/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/biom16020203","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41750274","host_type":"repository"},{"url":"https://doaj.org/article/c3948957a4d04d23914eecbb14dcf92e","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12937744/","host_type":"repository"},{"url":"https://hdl.handle.net/21.11116/0000-0012-AC62-C","host_type":"repository"},{"url":"https://ruj.uj.edu.pl/handle/item/570651","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12937744","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12937744?pdf=render","host_type":"Europe_PMC"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41750274/","host_type":""},{"url":"https://hdl.handle.net/21.11116/0000-0012-AC64-A","host_type":""}],"fields_of_study":["Telomeres, Telomerase, and Senescence","Genomics and Chromatin Dynamics","Fungal and yeast genetics research","0301 basic medicine","03 medical and health sciences","Animals","Cell Nucleus","Humans","Mammals","Yeasts","Chromatin","Chromatin Assembly and Disassembly","Transcription, Genetic"],"mesh_terms":["Animals","Cell Nucleus","Chromatin","Humans","Mammals","Transcription, Genetic","Yeasts","Chromatin Assembly and Disassembly"],"keywords":["Chromatin","Multicellular organism","Transcription factor","Intron","Histone","Reprogramming","Transcription (linguistics)","Gene","Cell nucleus","Chromatin remodeling","Post-transcriptional regulation","Epigenetic regulation","Nuclear Organization","Transcriptional Reprogramming","Cellular Quiescence","Mammals","Yeasts","Animals","Humans","Review"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"geo"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T03:17:19.467090Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}