{"doi":"10.3390/biom12111584","title":"Submilligram Level of Beetle Antifreeze Proteins Minimize Cold-Induced Cell Swelling and Promote Cell Survival","abstract":"Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypothermia-induced cell swelling. Antifreeze proteins (AFPs) found in many cold-adapted organisms can prevent cold injury of the organisms. Here, the effects of a beetle AFP from Dendroides canadensis (DAFP-1) on pancreatic β-cells preservation were first investigated. As low as 500 µg/mL, DAFP-1 significantly minimized INS-1 cell swelling and subsequent cell death during 4 °C preservation in UW solution for up to three days. However, such significant cytoprotection was not observed by an AFP from Tenebrio molitor (TmAFP), a structural homologue to DAFP-1 but lacking arginine, at the same levels. The cytoprotective effect of DAFP-1 was further validated with the primary β-cells in the isolated rat pancreatic islets in UW solution. The submilligram level supplement of DAFP-1 to UW solution significantly increased the islet mass recovery after three days of cold preservation followed by rewarming. The protective effects of DAFP-1 in UW solution were discussed at a molecular level. The results indicate the potential of DAFP-1 to enhance cell survival during extended cold preservation.","journal":"Biomolecules","year":2022,"id":296011,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9482,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":982756,"name":"Ignacio González","orcid":null,"position":1,"is_corresponding":false},{"id":922374,"name":"Cindy Nguyen","orcid":null,"position":2,"is_corresponding":false},{"id":982205,"name":"Shanti N. Raminani","orcid":"0000-0003-1305-1045","position":3,"is_corresponding":false},{"id":982757,"name":"Victor M. Deleon","orcid":null,"position":4,"is_corresponding":false},{"id":922375,"name":"Pedro Meza","orcid":null,"position":5,"is_corresponding":false},{"id":982758,"name":"Jose Zamalloa","orcid":null,"position":6,"is_corresponding":false},{"id":982206,"name":"Rachel Perez","orcid":"0000-0002-4274-1128","position":7,"is_corresponding":false},{"id":941516,"name":"Nelson Gonzalez","orcid":null,"position":8,"is_corresponding":false},{"id":940968,"name":"Hirotake Komatsu","orcid":"0000-0003-0876-4809","position":9,"is_corresponding":false},{"id":743286,"name":"Ismail H. Al-Abdullah","orcid":"0000-0001-7567-0453","position":10,"is_corresponding":false},{"id":921928,"name":"Xin Wen","orcid":"0000-0002-2465-7870","position":11,"is_corresponding":false},{"id":743285,"name":"Keiko Omori","orcid":"0000-0002-4200-8460","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-19T00:31:12.531553Z","pmid":"36358934","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}