{"doi":"10.3390/antiox12020361","title":"Green Synthesized Zinc Oxide Nanoparticles Using Moringa olifera Ethanolic Extract Lessens Acrylamide-Induced Testicular Damage, Apoptosis, and Steroidogenesis-Related Gene Dysregulation in Adult Rats","abstract":"leaf extract (MO-ZNPs) in acrylamide (ACR)-induced reproductive dysfunctions in male rats. ACR (20 mg/kg b.wt/day) and/or MO-ZNPs (10 mg/kg b.wt/day) were given orally by gastric gavage for 60 days. Then, sperm parameters; testicular enzymes; oxidative stress markers; reproductive hormones including testosterone, luteinizing hormone (LH)-estradiol, and follicle-stimulating hormone (FSH) concentration; testis histology; steroidogenesis-related gene expression; and apoptotic markers were examined. The findings revealed that MO-ZNPs significantly ameliorated the ACR-induced decline in the gonadosomatic index and altered the pituitary-gonadal axis, reflected by decreased serum testosterone and FSH with increased estradiol and LH, and sperm analysis disruption. Furthermore, a notable restoration of the tissue content of antioxidants (catalase and reduced glutathione) but depletion of malondialdehyde was evident in MO-ZNPs+ACR-treated rats compared to ACR-exposed ones. In addition, MO-ZNPs oral dosing markedly rescued the histopathological changes and apoptotic caspase-3 reactions in the testis resulting from ACR exposure. Furthermore, in MO-ZNPs+ACR-treated rats, ACR-induced downregulation of testicular steroidogenesis genes and proliferating cell nuclear antigen (PCNA) immune-expression were reversed. Conclusively, MO-ZNPs protected male rats from ACR-induced reproductive toxicity by suppressing oxidative injury and apoptosis while boosting steroidogenesis and sex hormones.","journal":"Antioxidants","year":2023,"id":323576,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9519,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1038470,"name":"Amany Behairy","orcid":"0000-0002-4962-7096","position":1,"is_corresponding":false},{"id":1038471,"name":"Yasmina M. Abd‐Elhakim","orcid":"0000-0002-3646-6385","position":2,"is_corresponding":false},{"id":1038472,"name":"Amany Abdel-Rahman Mohamed","orcid":"0000-0003-0061-2675","position":3,"is_corresponding":false},{"id":1038473,"name":"Ahmed E. Noreldin","orcid":"0000-0002-8344-0807","position":4,"is_corresponding":false},{"id":1038474,"name":"Naief Dahran","orcid":"0000-0003-3560-8166","position":5,"is_corresponding":false},{"id":1038836,"name":"Rasha A. Gaber","orcid":null,"position":6,"is_corresponding":false},{"id":1038475,"name":"Leena S. Alqahtani","orcid":"0000-0003-1789-2239","position":7,"is_corresponding":false},{"id":1038476,"name":"Walaa M. Essawi","orcid":"0000-0002-3212-1400","position":8,"is_corresponding":false},{"id":1038477,"name":"Areej A. Eskandrani","orcid":"0000-0002-2488-5400","position":9,"is_corresponding":false},{"id":1038837,"name":"Eman S. El‐Shetry","orcid":null,"position":10,"is_corresponding":false},{"id":1038469,"name":"Gomaa Mostafa‐Hedeab","orcid":"0000-0003-4947-0495","position":0,"is_corresponding":true}],"reference_count":122,"raw_metadata":null,"created_at":"2026-07-19T01:07:52.377944Z","pmid":"36829920","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}