{"doi":"10.3390/antib14030072","title":"Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells","abstract":"<jats:p>Background: Monoclonal antibodies play an important role in therapeutic and analytical applications. For recombinant expression, the coding sequences of the variable regions of the heavy and light chains are required. In addition, cloning antibody sequences, including constant regions, reduces the impact of hybridoma cell loss and ensures preservation of the naturally occurring full antibody sequence. Method: We combined amplification of IgG antibody variable regions from hybridoma mRNA with an advanced method for full-length cloning of monoclonal antibodies in a simple two-step workflow. Following Sanger sequencing and evaluation of consensus sequences, the best matching variable, diversity, and joining (V-(D-)J) gene segments were identified according to identity scores from IgBLAST reference sequences. Simultaneously, the mouse IgG subclass was determined at the DNA level based on isotype-specific sequence patterns in the CH1 domain. Knowing the DNA sequence of V-(D-)J recombination responsible for the complementary determining region 3 (CDR 3), variable region-specific primers were designed and used to amplify the corresponding antibody constant regions. Results: To verify the approach, we applied it to the hybridoma clone BAM-CCMV-29-81 and obtained identical full-length antibody sequences as with RNA Illumina sequencing. Further validation at the protein level using an established MALDI-TOF MS-fingerprinting protocol showed that five out of six genetically encoded CDR domains of the monoclonal antibody BAM-CCMV-29-81 could be efficiently correlated. Conclusion: This simple, streamlined method enables the cost-effective determination of the full-length sequence of monoclonal antibodies from hybridoma cell lines, with the added benefit of obtaining the DNA sequence of the antibody ready for recombinant expression.</jats:p>","journal":"Antibodies","year":2025,"id":625069,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1616148,"name":"Georg Tscheuschner","orcid":"0000-0002-2311-1016","position":1,"is_corresponding":false},{"id":1616149,"name":"Sabine Flemig","orcid":null,"position":2,"is_corresponding":false},{"id":1616150,"name":"Michael G. Weller","orcid":"0000-0003-2767-2029","position":3,"is_corresponding":false},{"id":1616151,"name":"Zoltán Konthur","orcid":"0000-0002-8767-9823","position":4,"is_corresponding":false},{"id":1616147,"name":"Sarah Döring","orcid":"0009-0005-9610-7041","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells","abstract":"<jats:p>Background: Monoclonal antibodies play an important role in therapeutic and analytical applications. For recombinant expression, the coding sequences of the variable regions of the heavy and light chains are required. In addition, cloning antibody sequences, including constant regions, reduces the impact of hybridoma cell loss and ensures preservation of the naturally occurring full antibody sequence. Method: We combined amplification of IgG antibody variable regions from hybridoma mRNA with an advanced method for full-length cloning of monoclonal antibodies in a simple two-step workflow. Following Sanger sequencing and evaluation of consensus sequences, the best matching variable, diversity, and joining (V-(D-)J) gene segments were identified according to identity scores from IgBLAST reference sequences. Simultaneously, the mouse IgG subclass was determined at the DNA level based on isotype-specific sequence patterns in the CH1 domain. Knowing the DNA sequence of V-(D-)J recombination responsible for the complementary determining region 3 (CDR 3), variable region-specific primers were designed and used to amplify the corresponding antibody constant regions. Results: To verify the approach, we applied it to the hybridoma clone BAM-CCMV-29-81 and obtained identical full-length antibody sequences as with RNA Illumina sequencing. Further validation at the protein level using an established MALDI-TOF MS-fingerprinting protocol showed that five out of six genetically encoded CDR domains of the monoclonal antibody BAM-CCMV-29-81 could be efficiently correlated. Conclusion: This simple, streamlined method enables the cost-effective determination of the full-length sequence of monoclonal antibodies from hybridoma cell lines, with the added benefit of obtaining the DNA sequence of the antibody ready for recombinant expression.</jats:p>","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40981271","pmcid":"PMC12452578","openalex_id":"https://openalex.org/W4413434247","authors":[],"funders":[],"total_grants":0,"fwci":0.4238,"citation_percentile":0.61827153,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2073-4468/14/3/72/pdf?version=1755846035","host_type":"journal"},{"url":"https://www.mdpi.com/2073-4468/14/3/72/pdf?version=1755846035","host_type":"publisher"},{"url":"https://www.mdpi.com/2073-4468/14/3/72/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/antib14030072","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40981271","host_type":"repository"},{"url":"https://doaj.org/article/9c12ef660513402bae2112cdb7c4ec15","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12452578","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12452578/","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12452578","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12452578?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Viral Infectious Diseases and Gene Expression in Insects","Monoclonal and Polyclonal Antibodies Research","Microfluidic and Capillary Electrophoresis Applications"],"mesh_terms":[],"keywords":["Monoclonal antibody","Computational biology","Molecular biology","Antibody","Biology","Virology","Chemistry","Computer science","Genetics","MALDI-TOF MS","Rna Illumina Sequencing","Full-length Antibody Sequencing","Hybridoma Cell Loss","Immunoglobulin Isotyping"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T05:43:08.259241Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}