{"doi":"10.3389/fpubh.2021.744535","title":"Application of SARS-CoV-2 Serology to Address Public Health Priorities","abstract":"Background: Antibodies against SARS-CoV-2 can be detected by various testing platforms, but a detailed understanding of assay performance is critical. Methods: We developed and validated a simple enzyme-linked immunosorbent assay (ELISA) to detect IgG binding to the receptor-binding domain (RBD) of SARS-CoV-2, which was then applied for surveillance. ELISA results were compared to a set of complimentary serologic assays using a large panel of clinical research samples. Results: The RBD ELISA exhibited robust performance in ROC curve analysis (AUC> 0.99; Se = 89%, Sp = 99.3%). Antibodies were detected in 23/353 (6.5%) healthcare workers, 6/9 RT-PCR-confirmed mild COVID-19 cases, and 0/30 non-COVID-19 cases from an ambulatory site. RBD ELISA showed a positive correlation with neutralizing activity ( p = <0.0001, R 2 = 0.26). Conclusions: We applied a validated SARS-CoV-2-specific IgG ELISA in multiple contexts and performed orthogonal testing on samples. This study demonstrates the utility of a simple serologic assay for detecting prior SARS-CoV-2 infection, particularly as a tool for efficiently testing large numbers of samples as in population surveillance. Our work also highlights that precise understanding of SARS-CoV-2 infection and immunity at the individual level, particularly with wide availability of vaccination, may be improved by orthogonal testing and/or more complex assays such as multiplex bead assays.","journal":"Frontiers in Public Health","year":2021,"id":207962,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9567,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":731184,"name":"Teresa Smith","orcid":"0000-0002-1792-2664","position":1,"is_corresponding":false},{"id":731863,"name":"Yerun Zhu","orcid":null,"position":2,"is_corresponding":false},{"id":338674,"name":"Kaitlin R. Taibl","orcid":"0000-0003-3828-2544","position":3,"is_corresponding":false},{"id":585458,"name":"Jessica Howard‐Anderson","orcid":"0000-0001-9924-0458","position":4,"is_corresponding":false},{"id":794522,"name":"Taylor Landay","orcid":null,"position":5,"is_corresponding":false},{"id":237958,"name":"Nora Pisanic","orcid":"0000-0002-0042-9232","position":6,"is_corresponding":false},{"id":384284,"name":"Jennifer Kleinhenz","orcid":null,"position":7,"is_corresponding":false},{"id":794007,"name":"Trevor W. Simon","orcid":"0000-0001-9930-1011","position":8,"is_corresponding":false},{"id":731862,"name":"Daniel Espinoza","orcid":null,"position":9,"is_corresponding":false},{"id":731861,"name":"Neena Edupuganti","orcid":null,"position":10,"is_corresponding":false},{"id":794523,"name":"Skyler Hammond","orcid":null,"position":11,"is_corresponding":false},{"id":104987,"name":"Nadine Rouphael","orcid":"0000-0002-2512-7919","position":12,"is_corresponding":false},{"id":757487,"name":"Huifeng Shen","orcid":"0000-0002-8885-8778","position":13,"is_corresponding":false},{"id":237965,"name":"Jessica K. Fairley","orcid":"0000-0003-4086-9272","position":14,"is_corresponding":false},{"id":106174,"name":"Srilatha Edupuganti","orcid":"0000-0001-6566-9969","position":15,"is_corresponding":false},{"id":794008,"name":"Jaime A. Cardona‐Ospina","orcid":"0000-0003-3996-2293","position":16,"is_corresponding":false},{"id":794009,"name":"Alfonso J. Rodríguez‐Morales","orcid":"0000-0001-9773-2192","position":17,"is_corresponding":false},{"id":103773,"name":"Lakshmanane Premkumar","orcid":"0000-0002-1736-1350","position":18,"is_corresponding":false},{"id":226174,"name":"Jens Wrammert","orcid":"0000-0002-1733-4424","position":19,"is_corresponding":false},{"id":343808,"name":"Rick L. Tarleton","orcid":"0000-0002-9589-5243","position":20,"is_corresponding":false},{"id":271489,"name":"Scott K. Fridkin","orcid":"0000-0003-4892-693X","position":21,"is_corresponding":false},{"id":237969,"name":"Christopher D. Heaney","orcid":"0000-0003-3211-8495","position":22,"is_corresponding":false},{"id":226165,"name":"Erin M. Scherer","orcid":"0000-0002-8794-1932","position":23,"is_corresponding":false},{"id":237968,"name":"Matthew H. Collins","orcid":"0000-0001-7974-1933","position":24,"is_corresponding":false},{"id":237966,"name":"Amy C Sherman","orcid":"0000-0002-6075-3990","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-18T23:51:53.662668Z","pmid":"34888282","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}