{"doi":"10.3389/fpls.2022.928386","title":"The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses","abstract":"The accumulation of the auxin precursor indole-3-acetamide (IAM) in the <i>ami1</i> mutant has recently been reported to reduce plant growth and to trigger abiotic stress responses in <i>Arabidopsis thaliana</i>. The observed response includes the induction of abscisic acid (ABA) biosynthesis through the promotion of <i>NCED3</i> expression. The mechanism by which plant growth is limited, however, remained largely unclear. Here, we investigated the transcriptional responses evoked by the exogenous application of IAM using comprehensive RNA-sequencing (RNA-seq) and reverse genetics approaches. The RNA-seq results highlighted the induction of a small number of genes, including the R2R3 MYB transcription factor genes <i>MYB74</i> and <i>MYB102</i>. The two MYB factors are known to respond to various stress cues and to ABA. Consistent with a role as negative plant growth regulator, conditional <i>MYB74</i> overexpressor lines showed a considerable growth reduction. RNA-seq analysis of <i>MYB74</i> mutants indicated an association of MYB74 with responses to osmotic stress, water deprivation, and seed development, which further linked MYB74 with the observed <i>ami1</i> osmotic stress and seed phenotype. Collectively, our findings point toward a role for MYB74 in plant growth control and in responses to abiotic stress stimuli.","journal":"Frontiers in Plant Science","year":2022,"id":5166,"datarank":0.9319948103789066,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.39446696961049016,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.39446696961049016,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":29,"citers_with_citation_signal":20,"citers_with_endowment":20,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.052,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-06-22","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":22062,"name":"Marta-Marina Pérez-Alonso","orcid":null,"position":1,"is_corresponding":false},{"id":52199,"name":"Adrián González Ortega-Villaizán","orcid":null,"position":2,"is_corresponding":false},{"id":52200,"name":"Beatriz Sánchez-Parra","orcid":null,"position":3,"is_corresponding":false},{"id":52201,"name":"Jutta Ludwig-Müller","orcid":null,"position":4,"is_corresponding":false},{"id":606,"name":"Mark D D. Wilkinson","orcid":"0000-0001-6960-357X","position":5,"is_corresponding":false},{"id":22066,"name":"Stephan Pollmann","orcid":"0000-0002-5111-4425","position":6,"is_corresponding":false},{"id":52202,"name":"Paloma Ortiz‐García","orcid":"0000-0001-5007-4188","position":7,"is_corresponding":false},{"id":22067,"name":"Marta‐Marina Pérez‐Alonso","orcid":"0000-0003-4118-4822","position":8,"is_corresponding":false},{"id":52203,"name":"Adrián González Ortega‐Villaizán","orcid":"0000-0001-8221-6793","position":9,"is_corresponding":false},{"id":52204,"name":"Beatriz Sánchez‐Parra","orcid":"0000-0002-3585-3201","position":10,"is_corresponding":false},{"id":52205,"name":"Jutta Ludwig‐Müller","orcid":"0000-0002-9403-5153","position":11,"is_corresponding":false},{"id":52198,"name":"Paloma Ortiz-García","orcid":null,"position":0,"is_corresponding":true}],"reference_count":76,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}