{"doi":"10.3389/fpls.2022.837517","title":"Sulfur Stable Isotope Discrimination in Rice: A Sulfur Isotope Mass Balance Study","abstract":"<jats:p>The use of sulfur (S) stable isotopes to study S metabolism in plants is still limited by the relatively small number of studies. It is generally accepted that less S stable isotope discrimination occurs during sulfate (SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup>) uptake. However, S metabolism and allocation are expected to produce separations of S stable isotopes among the different plant S pools and organs. In this study, we measured the S isotope composition of the main S pools of rice plants grown under different SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> availabilities in appropriate closed and open hydroponic-plant systems. The main results indicate that fractionation against <jats:sup>34</jats:sup>S occurred during SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> uptake. Fractionation was dependent on the amount of residual SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> in the solution, showing a biphasic behavior related to the relative expression of two SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> transporter genes (<jats:italic>OsSULTR1;1</jats:italic> and <jats:italic>OsSULTR1;2</jats:italic>) in the roots. S isotope separations among S pools and organs were also observed as the result of substantial S isotope fractionations and mixing effects occurring during SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> assimilation and plant S partitioning. Since the S stable isotope separations conserve the memory of the physiological and metabolic activities that determined them, we here underline the potential of the <jats:sup>32</jats:sup>S/<jats:sup>34</jats:sup>S analysis for the detailed characterization of the metabolic and molecular processes involved in plant S nutrition and homeostasis.</jats:p>","journal":"Frontiers in Plant Science","year":2022,"id":679263,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1774753,"name":"Moez Maghrebi","orcid":null,"position":1,"is_corresponding":false},{"id":1774754,"name":"Mariachiara Caschetto","orcid":null,"position":2,"is_corresponding":false},{"id":1774755,"name":"Gian Attilio Sacchi","orcid":null,"position":3,"is_corresponding":false},{"id":1774756,"name":"Fabio Francesco Nocito","orcid":null,"position":4,"is_corresponding":false},{"id":1774752,"name":"Viviana Cavallaro","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sulfur Stable Isotope Discrimination in Rice: A Sulfur Isotope Mass Balance Study","abstract":"<jats:p>The use of sulfur (S) stable isotopes to study S metabolism in plants is still limited by the relatively small number of studies. It is generally accepted that less S stable isotope discrimination occurs during sulfate (SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup>) uptake. However, S metabolism and allocation are expected to produce separations of S stable isotopes among the different plant S pools and organs. In this study, we measured the S isotope composition of the main S pools of rice plants grown under different SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> availabilities in appropriate closed and open hydroponic-plant systems. The main results indicate that fractionation against <jats:sup>34</jats:sup>S occurred during SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> uptake. Fractionation was dependent on the amount of residual SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> in the solution, showing a biphasic behavior related to the relative expression of two SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> transporter genes (<jats:italic>OsSULTR1;1</jats:italic> and <jats:italic>OsSULTR1;2</jats:italic>) in the roots. S isotope separations among S pools and organs were also observed as the result of substantial S isotope fractionations and mixing effects occurring during SO<jats:sub>4</jats:sub><jats:sup>2–</jats:sup> assimilation and plant S partitioning. Since the S stable isotope separations conserve the memory of the physiological and metabolic activities that determined them, we here underline the potential of the <jats:sup>32</jats:sup>S/<jats:sup>34</jats:sup>S analysis for the detailed characterization of the metabolic and molecular processes involved in plant S nutrition and homeostasis.</jats:p>","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35360342","pmcid":"PMC8960986","openalex_id":"https://openalex.org/W4220693979","authors":[],"funders":[],"total_grants":0,"fwci":0.3915,"citation_percentile":0.52922359,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.3389/fpls.2022.837517","host_type":"journal"},{"url":"https://doi.org/10.3389/fpls.2022.837517","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fpls.2022.837517/full","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35360342","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8960986","host_type":"repository"},{"url":"https://doaj.org/article/c65a46aa0f7d4881bf92ad2223e599e2","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8960986","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8960986?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Nitrogen and Sulfur Effects on Brassica","Plant nutrient uptake and metabolism","Selenium in Biological Systems"],"mesh_terms":[],"keywords":["Isotope","Sulfur","δ34S","Stable isotope ratio","Fractionation","Isotope fractionation","Isotope analysis","Chemistry","Sulfate","Isotopes of sulfur","Assimilation (phonology)","Environmental chemistry","Botany","Biology","Ecology","Biochemistry","Chromatography","Oryza sativa L.","Sulfur Assimilation","Sulfate Uptake","Sulfur Stable Isotopes"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T12:42:55.566814Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}