{"doi":"10.3389/fphys.2025.1698399","title":"Entropy as a marker of physiological transition during pediatric cardiopulmonary exercise testing","abstract":"This research analyzed the sample entropy (SampEn) of breath-by-breath cardiopulmonary exercise testing (CPET) data from 170 healthy pediatric participants (85 males) 8 to 18-years-old, using a Bayesian statistics approach. SampEn measures the complexity of time series data, providing quantitative insight into the predictability of breathing patterns in pediatric participants. To address non-stationarity, signals were differenced prior to SampEn calculation. In addition to sex and age group comparisons, we examined SampEn before and after the midpoint of each participant’s CPET to assess how SampEn changes as exercise intensity increases. We corroborated previous findings that SampEn decreases in the later half of CPET for healthy pediatric participants for oxygen uptake <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m1\"> <mml:mrow> <mml:mo stretchy=\"false\">(</mml:mo> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:mi>V</mml:mi> </mml:mrow> <mml:mo>̇</mml:mo> </mml:mover> </mml:mrow> <mml:msub> <mml:mrow> <mml:mi>O</mml:mi> </mml:mrow> <mml:mrow> <mml:mn>2</mml:mn> </mml:mrow> </mml:msub> </mml:mrow> <mml:mo stretchy=\"false\">)</mml:mo> </mml:mrow> </mml:math> , carbon dioxide output <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m2\"> <mml:mrow> <mml:mo stretchy=\"false\">(</mml:mo> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:mi>V</mml:mi> </mml:mrow> <mml:mo>̇</mml:mo> </mml:mover> </mml:mrow> <mml:mi>C</mml:mi> <mml:msub> <mml:mrow> <mml:mi>O</mml:mi> </mml:mrow> <mml:mrow> <mml:mn>2</mml:mn> </mml:mrow> </mml:msub> </mml:mrow> <mml:mo stretchy=\"false\">)</mml:mo> </mml:mrow> </mml:math> , ventilation <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m3\"> <mml:mrow> <mml:mo stretchy=\"false\">(</mml:mo> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:mi>V</mml:mi> </mml:mrow> <mml:mo>̇</mml:mo> </mml:mover> </mml:mrow> <mml:mi>E</mml:mi> </mml:mrow> <mml:mo stretchy=\"false\">)</mml:mo> </mml:mrow> </mml:math> , and heart rate <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m4\"> <mml:mrow> <mml:mo stretchy=\"false\">(</mml:mo> <mml:mrow> <mml:mi>H</mml:mi> <mml:mi>R</mml:mi> </mml:mrow> <mml:mo stretchy=\"false\">)</mml:mo> </mml:mrow> </mml:math> . Females tended to have higher SampEn than their male counterparts, with a statistically significant difference between the sexes in older participants for <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m5\"> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:mi>V</mml:mi> </mml:mrow> <mml:mo>̇</mml:mo> </mml:mover> </mml:mrow> <mml:msub> <mml:mrow> <mml:mi>O</mml:mi> </mml:mrow> <mml:mrow> <mml:mn>2</mml:mn> </mml:mrow> </mml:msub> </mml:mrow> </mml:math> , <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m6\"> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:mi>V</mml:mi> </mml:mrow> <mml:mo>̇</mml:mo> </mml:mover> </mml:mrow> <mml:mi>C</mml:mi> <mml:msub> <mml:mrow> <mml:mi>O</mml:mi> </mml:mrow> <mml:mrow> <mml:mn>2</mml:mn> </mml:mrow> </mml:msub> </mml:mrow> </mml:math> , <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m7\"> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:mi>V</mml:mi> </mml:mrow> <mml:mo>̇</mml:mo> </mml:mover> </mml:mrow> <mml:mi>E</mml:mi> </mml:mrow> </mml:math> , <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m8\"> <mml:mrow> <mml:mi>H</mml:mi> <mml:mi>R</mml:mi> </mml:mrow> </mml:math> , and respiratory rate <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m9\"> <mml:mrow> <mml:mo stretchy=\"false\">(</mml:mo> <mml:mrow> <mml:mi>R</mml:mi> <mml:mi>R</mml:mi> </mml:mrow> <mml:mo stretchy=\"false\">)</mml:mo> </mml:mrow> </mml:math> . Age-related findings included: significantly higher SampEn in younger males compared to older males for <mml:math xmlns:mml=\"http://www.w3.org/1998/Math/MathML\" id=\"m10\"> <mml:mrow> <mml:mrow> <mml:mover accent=\"true\"> <mml:mrow> <mml:","journal":"Frontiers in Physiology","year":2025,"id":583676,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9216,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":315122,"name":"Donald Brown","orcid":"0000-0002-9140-2632","position":1,"is_corresponding":false},{"id":1315785,"name":"D. 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Cooper","orcid":"0000-0003-1736-775X","position":2,"is_corresponding":false},{"id":811158,"name":"Annamarie Stehli","orcid":"0000-0002-7985-1726","position":3,"is_corresponding":false},{"id":811787,"name":"Shlomit Aizik","orcid":null,"position":4,"is_corresponding":false},{"id":1496640,"name":"Natalie Kupperman","orcid":null,"position":5,"is_corresponding":false},{"id":1496639,"name":"Kaleigh O’Hara","orcid":null,"position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-19T02:59:07.970345Z","pmid":"41403468","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}