{"doi":"10.3389/fphys.2023.1167904","title":"The role of dietary magnesium deficiency in inflammatory hypertension","abstract":"Nearly 30% of adults consume less than the estimated average daily requirement of magnesium (Mg 2+ ), and commonly used medications, such as diuretics, promote Mg 2+ deficiency. Higher serum Mg 2+ levels, increased dietary Mg 2+ in-take, and Mg 2+ supplementation are each associated with lower blood pressure, suggesting that Mg 2+ -deficiency contributes to the pathogenesis of hypertension. Antigen-presenting cells, such as monocytes and dendritic cells, are well-known to be involved in the pathogenesis of hypertension. In these cells, processes implicated as necessary for increased blood pressure include activation of the NLRP3 inflammasome, IL-1β production, and oxidative modification of fatty acids such as arachidonic acid, forming isolevuglandins (IsoLGs). We hypothesized that increased blood pressure in response to dietary Mg 2+ -depletion leads to increased NLRP3, IL-1β, and IsoLG production in antigen presenting cells. We found that a Mg 2+ -depleted diet (0.01% Mg 2+ diet) increased blood pressure in mice compared to mice fed a 0.08% Mg 2+ diet. Mg 2+ -depleted mice did not exhibit an increase in total body fluid, as measured by quantitative magnetic resonance. Plasma IL-1β concentrations were increased (0.13 ± 0.02 pg/mL vs. 0.04 ± 0.02 pg/mL). Using flow cytometry, we observed increased NLRP3 and IL-1β expression in antigen-presenting cells from spleen, kidney, and aorta. We also observed increased IsoLG production in antigen-presenting cells from these organs. Primary culture of CD11c+ dendritic cells confirmed that low extracellular Mg 2+ exerts a direct effect on these cells, stimulating IL-1β and IL-18 production. The present findings show that NLRP3 inflammasome activation and IsoLG-adduct formation are stimulated when dietary Mg 2+ is depleted. Interventions and increased dietary Mg 2+ consumption may prove beneficial in decreasing the prevalence of hypertension and cardiovascular disease.","journal":"Frontiers in Physiology","year":2023,"id":339756,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9569,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1006915,"name":"Linh Huynh","orcid":"0000-0003-0540-6811","position":1,"is_corresponding":false},{"id":1073733,"name":"Ritam Patel","orcid":"0000-0002-6159-889X","position":2,"is_corresponding":false},{"id":1002007,"name":"Tracey Lam","orcid":null,"position":3,"is_corresponding":false},{"id":380646,"name":"Daniel J. Bain","orcid":"0000-0003-1979-7016","position":4,"is_corresponding":false},{"id":1062410,"name":"Sydney Jamison","orcid":"0000-0003-2128-6303","position":5,"is_corresponding":false},{"id":265986,"name":"Annet Kirabo","orcid":"0000-0001-8580-9359","position":6,"is_corresponding":false},{"id":351626,"name":"Evan C. Ray","orcid":"0000-0003-1491-4869","position":7,"is_corresponding":false},{"id":864994,"name":"Ashley Pitzer Mutchler","orcid":"0000-0002-5478-3886","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T01:10:49.535464Z","pmid":"37293263","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}