{"doi":"10.3389/fphar.2023.1337319","title":"Utilizing multimodal mass spectrometry imaging for profiling immune cell composition and N-glycosylation across colorectal carcinoma disease progression","abstract":"Colorectal cancer (CRC) stands as a leading cause of death worldwide, often arising from specific genetic mutations, progressing from pre-cancerous adenomas to adenocarcinomas. Early detection through regular screening can result in a 90% 5-year survival rate for patients. However, unfortunately, only a fraction of CRC cases are identified at pre-invasive stages, allowing progression to occur silently over 10–15 years. The intricate interplay between the immune system and tumor cells within the tumor microenvironment plays a pivotal role in the progression of CRC. Immune cell clusters can either inhibit or facilitate tumor initiation, growth, and metastasis. To gain a better understanding of this relationship, we conducted N-glycomic profiling using matrix-assisted laser desorption-ionization mass spectrometry imaging (MALDI-MSI). We detected nearly 100 N-glycan species across all samples, revealing a shift in N-glycome profiles from normal to cancerous tissues, marked by a decrease in high mannose N-glycans. Further analysis of precancerous to invasive carcinomas showed an increase in pauci-mannose biantennary, and tetraantennary N-glycans with disease progression. Moreover, a distinct stratification in the N-glycome profile was observed between non-mucinous and mucinous CRC tissues, driven by pauci-mannose, high mannose, and bisecting N-glycans. Notably, we identified immune clusters of CD20 + B cells and CD3/CD44+ T cells distinctive and predictive with signature profiles of bisecting and branched N-glycans. These spatial N-glycan profiles offer potential biomarkers and therapeutic targets throughout the progression of CRC.","journal":"Frontiers in Pharmacology","year":2024,"id":433492,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":406400,"name":"Paul J. Nietert","orcid":"0000-0002-3933-4986","position":1,"is_corresponding":false},{"id":930027,"name":"Rachel Stubler","orcid":"0000-0003-1487-1715","position":2,"is_corresponding":false},{"id":1239189,"name":"Caroline Kittrell","orcid":"0000-0002-2506-919X","position":3,"is_corresponding":false},{"id":692943,"name":"Grace Grimsley","orcid":null,"position":4,"is_corresponding":false},{"id":281071,"name":"David Lewin","orcid":"0000-0002-0531-6018","position":5,"is_corresponding":false},{"id":262766,"name":"Anand S. Mehta","orcid":"0000-0002-9846-9389","position":6,"is_corresponding":false},{"id":1239764,"name":"Chadi Hajar","orcid":null,"position":7,"is_corresponding":false},{"id":1114573,"name":"Katherine Wang","orcid":"0000-0002-0849-8443","position":8,"is_corresponding":false},{"id":639325,"name":"Elizabeth C. O’Quinn","orcid":null,"position":9,"is_corresponding":false},{"id":377097,"name":"Peggi M. Angel","orcid":"0000-0002-4436-555X","position":10,"is_corresponding":false},{"id":409378,"name":"Kristin Wallace","orcid":"0000-0002-4297-9064","position":11,"is_corresponding":false},{"id":281073,"name":"Richard R. Drake","orcid":"0000-0002-6285-6440","position":12,"is_corresponding":false},{"id":374601,"name":"Lyndsay E.A. Young","orcid":"0000-0002-6873-5674","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:59:49.434790Z","pmid":"38273829","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}