{"doi":"10.3389/fphar.2023.1284610","title":"Genome-wide CRISPR/Cas9 screening for drug resistance in tumors","abstract":"<jats:p>Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) screening is a simple screening method for locating loci under specific conditions, and it has been utilized in tumor drug resistance research for finding potential drug resistance-associated genes. This screening strategy has significant implications for further treatment of malignancies with acquired drug resistance. In recent years, studies involving genome-wide CRISPR/Cas9 screening have gradually increased. Here we review the recent application of genome-wide CRISPR/Cas9 screening for drug resistance, involving mitogen-activated protein kinase (MAPK) pathway inhibitors, poly (ADP-ribose) polymerase inhibitors (PARPi), alkylating agents, mitotic inhibitors, antimetabolites, immune checkpoint inhibitors (ICIs), and cyclin-dependent kinase inhibitors (CDKI). We summarize drug resistance pathways such as the KEAP1/Nrf2 pathway MAPK pathway, and NF-κB pathway. Also, we analyze the limitations and conditions for the application of genome-wide CRISPR/Cas9 screening techniques.</jats:p>","journal":"Frontiers in Pharmacology","year":2023,"id":607214,"datarank":0.4493598410330987,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.0,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":850610,"name":"Hailiang Wang","orcid":"0000-0003-4409-2034","position":1,"is_corresponding":false},{"id":1335284,"name":"Qian Yan","orcid":"0009-0008-7483-0572","position":2,"is_corresponding":false},{"id":1559107,"name":"Jinwei Cui","orcid":null,"position":3,"is_corresponding":false},{"id":1223913,"name":"Yubin Chen","orcid":"0000-0002-3566-5366","position":4,"is_corresponding":false},{"id":1559110,"name":"Shiye Ruan","orcid":null,"position":5,"is_corresponding":false},{"id":1559112,"name":"Jiayu Yang","orcid":null,"position":6,"is_corresponding":false},{"id":1559115,"name":"Zelong Wu","orcid":null,"position":7,"is_corresponding":false},{"id":1559117,"name":"Mingqian Han","orcid":null,"position":8,"is_corresponding":false},{"id":1559118,"name":"Shanzhou Huang","orcid":null,"position":9,"is_corresponding":false},{"id":226082,"name":"Qi Zhou","orcid":"0000-0002-7419-2047","position":10,"is_corresponding":false},{"id":246197,"name":"Chuanzhao Zhang","orcid":"0000-0001-6553-4458","position":11,"is_corresponding":false},{"id":246199,"name":"Baohua Hou","orcid":"0000-0002-4239-1367","position":12,"is_corresponding":false},{"id":1559106,"name":"Zhongyan Zhang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Genome-wide CRISPR/Cas9 screening for drug resistance in tumors","abstract":"<jats:p>Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) screening is a simple screening method for locating loci under specific conditions, and it has been utilized in tumor drug resistance research for finding potential drug resistance-associated genes. This screening strategy has significant implications for further treatment of malignancies with acquired drug resistance. In recent years, studies involving genome-wide CRISPR/Cas9 screening have gradually increased. Here we review the recent application of genome-wide CRISPR/Cas9 screening for drug resistance, involving mitogen-activated protein kinase (MAPK) pathway inhibitors, poly (ADP-ribose) polymerase inhibitors (PARPi), alkylating agents, mitotic inhibitors, antimetabolites, immune checkpoint inhibitors (ICIs), and cyclin-dependent kinase inhibitors (CDKI). We summarize drug resistance pathways such as the KEAP1/Nrf2 pathway MAPK pathway, and NF-κB pathway. Also, we analyze the limitations and conditions for the application of genome-wide CRISPR/Cas9 screening techniques.</jats:p>","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38084101","pmcid":"PMC10710899","openalex_id":"https://openalex.org/W4388946190","authors":[],"funders":[],"total_grants":0,"fwci":1.6852,"citation_percentile":0.84573132,"influential_citations":0,"citation_trend":[{"year":2024,"count":5},{"year":2025,"count":11},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.frontiersin.org/articles/10.3389/fphar.2023.1284610/pdf?isPublishedV2=False","host_type":"journal"},{"url":"https://www.frontiersin.org/articles/10.3389/fphar.2023.1284610/pdf?isPublishedV2=False","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fphar.2023.1284610/full","host_type":"publisher"},{"url":"https://doi.org/10.3389/fphar.2023.1284610","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38084101","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10710899","host_type":"repository"},{"url":"https://doaj.org/article/5060ad9332634fd6b6d1a3b4a5d34429","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10710899/pdf/fphar-14-1284610.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10710899","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10710899?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["CRISPR and Genetic Engineering","Cytomegalovirus and herpesvirus research","Cancer Mechanisms and Therapy"],"mesh_terms":[],"keywords":["CRISPR","Cas9","Biology","Drug resistance","Genome editing","Genome","Trans-activating crRNA","Computational biology","Drug discovery","Gene","Genetics","Bioinformatics","Tumors","Parp Inhibitors","Mapk Pathway Inhibitors","Genome-wide Crispr/cas9 Screening"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T05:58:46.332273Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}