{"doi":"10.3389/fped.2023.1308700","title":"The proteomic fingerprint in infants with single ventricle heart disease in the interstage period: evidence of chronic inflammation and widespread activation of biological networks","abstract":"Introduction: Children with single ventricle heart disease (SVHD) experience significant morbidity across systems and time, with 70% of patients experiencing acute kidney injury, 33% neurodevelopmental impairment, 14% growth failure, and 5.5% of patients suffering necrotizing enterocolitis. Proteomics is a method to identify new biomarkers and mechanisms of injury in complex physiologic states. Methods: Infants with SVHD in the interstage period were compared to similar-age healthy controls. Serum samples were collected, stored at -80°C, and run on a panel of 1,500 proteins in single batch analysis (Somalogic Inc., CO). Partial Least Squares-Discriminant Analysis (PLS-DA) was used to compare the proteomic profile of cases and controls and t-tests to detect differences in individual proteins (FDR <0.05). Protein network analysis with functional enrichment was performed in STRING and Cytoscape. Results: = 0.80). 568 proteins differed between groups (FDR <0.05). We identified 25 up-regulated functional clusters and 13 down-regulated. Active biological systems fell into six key groups: angiogenesis and cell proliferation/turnover, immune system activation and inflammation, altered metabolism, neural development, gastrointestinal system, and cardiac physiology and development. Conclusions: We report a clear differentiation in the circulating proteome of patients with SVHD and healthy controls with >500 circulating proteins distinguishing the groups. These proteomic data identify widespread protein dysregulation across multiple biologic systems with promising biological plausibility as drivers of SVHD morbidity.","journal":"Frontiers in Pediatrics","year":2023,"id":371899,"datarank":0.40938142623487006,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.11749490387657299,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.11749490387657299,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":4,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9578,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":443448,"name":"Christopher A Mancuso","orcid":"0000-0003-3081-2758","position":1,"is_corresponding":false},{"id":1131941,"name":"Kelly R. Wolfe","orcid":"0000-0003-4754-4577","position":2,"is_corresponding":false},{"id":386622,"name":"Ludmila Khailová","orcid":null,"position":3,"is_corresponding":false},{"id":1041813,"name":"Sierra Niemiec","orcid":"0000-0001-9868-0654","position":4,"is_corresponding":false},{"id":1132350,"name":"Eiman Ali","orcid":null,"position":5,"is_corresponding":false},{"id":1107861,"name":"Michael V. DiMaria","orcid":null,"position":6,"is_corresponding":false},{"id":949910,"name":"Max B. Mitchell","orcid":"0000-0002-8350-0646","position":7,"is_corresponding":false},{"id":797160,"name":"Mark Twite","orcid":"0000-0003-4204-4450","position":8,"is_corresponding":false},{"id":513325,"name":"Gareth J. Morgan","orcid":"0000-0003-1500-9334","position":9,"is_corresponding":false},{"id":481291,"name":"Benjamin S. Frank","orcid":"0000-0002-2227-6265","position":10,"is_corresponding":false},{"id":385601,"name":"Jesse Davidson","orcid":"0000-0001-9193-9803","position":11,"is_corresponding":false},{"id":1132349,"name":"Lindsay M. Thomson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":92,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:15:49.453761Z","pmid":"38143535","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}