{"doi":"10.3389/fnut.2022.846378","title":"Dynamics of the Gut Bacteria and Fungi Accompanying Low-Carbohydrate Diet-Induced Weight Loss in Overweight and Obese Adults","abstract":"<jats:sec><jats:title>Background</jats:title><jats:p>Low-carbohydrate (e.g., Atkins) dietary pattern is one of the most effective diets for weight loss, but little is known about the characteristics of the gut microbiota accompanying low-carbohydrate diets-induced weight loss. This study aims to profile dynamics of gut bacteria and fungi accompanying modified Atkins diets-induced weight loss among overweight and obese adults.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Overweight and obese adults were screened to follow a modified Atkins diet plan (30% of energy from protein, 40% from carbohydrate and 30% from fat). We longitudinally profiled dynamics of gut bacteria and fungi based on 16S rRNA and ITS rRNA gene sequencing data, respectively.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 65 participants followed the modified Atkins diets for 20–231 days, with 61 and 27 participants achieving a weight loss of at least 5 and 10%, respectively. Most of the participants who achieved 10% weight loss also experienced improvements on metabolic health. The diversity of gut bacteria and fungi increased after a weight loss of 5% and kept stable thereafter. Bacteria genera including <jats:italic>Lachnoclostridium</jats:italic> and <jats:italic>Ruminococcus 2</jats:italic> from <jats:italic>Firmicutes</jats:italic> phylum were depleted, while <jats:italic>Parabacteroides</jats:italic> and <jats:italic>Bacteroides</jats:italic> from <jats:italic>Bacteroidetes</jats:italic> phylum were enriched after weight loss. The inter-kingdom analysis found an intensive covariation between gut fungi and bacteria, involving more than half of the weight loss-associated bacteria.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>This study confirmed the modulation of bacterial and fungal composition during weight loss with the low-carbohydrate diets and showed previously unknown links between intestinal bacteria and fungi accompanying the weight loss.</jats:p></jats:sec>","journal":"Frontiers in Nutrition","year":2022,"id":647155,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1685900,"name":"Libin Xie","orcid":null,"position":1,"is_corresponding":false},{"id":395664,"name":"Wei Chen","orcid":"0000-0001-5451-6430","position":2,"is_corresponding":false},{"id":467841,"name":"Jin Qin","orcid":"0000-0002-9478-4218","position":3,"is_corresponding":false},{"id":490886,"name":"Jingjing Zhang","orcid":"0000-0002-1349-9184","position":4,"is_corresponding":false},{"id":1685901,"name":"Min Lei","orcid":null,"position":5,"is_corresponding":false},{"id":397875,"name":"Yue Wang","orcid":"0000-0001-6946-2666","position":6,"is_corresponding":false},{"id":1685902,"name":"Hongge Tang","orcid":null,"position":7,"is_corresponding":false},{"id":1685903,"name":"Sujuan Xue","orcid":null,"position":8,"is_corresponding":false},{"id":844097,"name":"Xinxiu Liang","orcid":"0009-0008-2895-4101","position":9,"is_corresponding":false},{"id":844101,"name":"Zelei Miao","orcid":"0000-0001-9478-1933","position":10,"is_corresponding":false},{"id":855241,"name":"Congmei Xiao","orcid":null,"position":11,"is_corresponding":false},{"id":1685904,"name":"Meishuang Shang","orcid":null,"position":12,"is_corresponding":false},{"id":434145,"name":"Jie Lu","orcid":"0000-0002-6982-2294","position":13,"is_corresponding":false},{"id":1685905,"name":"Hailing Di","orcid":null,"position":14,"is_corresponding":false},{"id":844098,"name":"Yuanqing Fu","orcid":"0000-0002-3955-9376","position":15,"is_corresponding":false},{"id":943420,"name":"Dan Yu","orcid":"0000-0001-7845-2574","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamics of the Gut Bacteria and Fungi Accompanying Low-Carbohydrate Diet-Induced Weight Loss in Overweight and Obese Adults","abstract":"<jats:sec><jats:title>Background</jats:title><jats:p>Low-carbohydrate (e.g., Atkins) dietary pattern is one of the most effective diets for weight loss, but little is known about the characteristics of the gut microbiota accompanying low-carbohydrate diets-induced weight loss. This study aims to profile dynamics of gut bacteria and fungi accompanying modified Atkins diets-induced weight loss among overweight and obese adults.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Overweight and obese adults were screened to follow a modified Atkins diet plan (30% of energy from protein, 40% from carbohydrate and 30% from fat). We longitudinally profiled dynamics of gut bacteria and fungi based on 16S rRNA and ITS rRNA gene sequencing data, respectively.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 65 participants followed the modified Atkins diets for 20–231 days, with 61 and 27 participants achieving a weight loss of at least 5 and 10%, respectively. Most of the participants who achieved 10% weight loss also experienced improvements on metabolic health. The diversity of gut bacteria and fungi increased after a weight loss of 5% and kept stable thereafter. Bacteria genera including <jats:italic>Lachnoclostridium</jats:italic> and <jats:italic>Ruminococcus 2</jats:italic> from <jats:italic>Firmicutes</jats:italic> phylum were depleted, while <jats:italic>Parabacteroides</jats:italic> and <jats:italic>Bacteroides</jats:italic> from <jats:italic>Bacteroidetes</jats:italic> phylum were enriched after weight loss. The inter-kingdom analysis found an intensive covariation between gut fungi and bacteria, involving more than half of the weight loss-associated bacteria.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>This study confirmed the modulation of bacterial and fungal composition during weight loss with the low-carbohydrate diets and showed previously unknown links between intestinal bacteria and fungi accompanying the weight loss.</jats:p></jats:sec>","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35223965","pmcid":"PMC8873986","openalex_id":"https://openalex.org/W4211135215","authors":[],"funders":[],"total_grants":0,"fwci":1.4912,"citation_percentile":0.81666658,"influential_citations":0,"citation_trend":[{"year":2022,"count":5},{"year":2023,"count":4},{"year":2024,"count":5},{"year":2025,"count":5},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.frontiersin.org/articles/10.3389/fnut.2022.846378/pdf","host_type":"journal"},{"url":"https://www.frontiersin.org/articles/10.3389/fnut.2022.846378/pdf","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fnut.2022.846378/full","host_type":"publisher"},{"url":"https://doi.org/10.3389/fnut.2022.846378","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35223965","host_type":"repository"},{"url":"https://doaj.org/article/f2dd78a869da413e94fa39f236ff0354","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8873986","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8873986","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8873986?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Gut microbiota and health","Diet and metabolism studies","Food composition and properties"],"mesh_terms":[],"keywords":["Weight loss","Firmicutes","Gut flora","Biology","Bacteria","Bacteroidetes","Food science","Carbohydrate","Bacteroides","Overweight","Obesity","Microbiology","16S ribosomal RNA","Endocrinology","Biochemistry","Genetics","Gut Bacteria","Low-carbohydrate Diet","Gut Fungi"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"nct"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T17:09:36.521586Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}