{"doi":"10.3389/fnsyn.2020.00006","title":"Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations","abstract":"Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent \"helper\" adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (ΔG) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.","journal":"Frontiers in Synaptic Neuroscience","year":2020,"id":58252,"datarank":1.910309243558918,"base_score":4.304065093204169,"endowment":4.304065093204169,"self_citation_contribution":0.6456097639806255,"citation_network_contribution":1.2646994795782924,"self_endowment_contribution":0.6456097639806255,"citer_contribution":1.2646994795782924,"corpus_percentile":null,"corpus_rank":null,"citation_count":73,"citer_count":57,"citers_with_citation_signal":45,"citers_with_endowment":45,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":12970,"name":"Lei Jin","orcid":"0000-0002-7264-4409","position":1,"is_corresponding":false},{"id":305048,"name":"Nicholas E. Lea","orcid":"0000-0002-8707-2976","position":2,"is_corresponding":false},{"id":12976,"name":"Ian R. Wickersham","orcid":"0000-0003-0389-5324","position":3,"is_corresponding":false},{"id":305047,"name":"Thomas K. Lavin","orcid":"0000-0003-0269-3325","position":0,"is_corresponding":true}],"reference_count":60,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:07:11.345501Z","pmid":"32116642","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}