{"doi":"10.3389/fnmol.2020.00149","title":"Detection of Synaptic Proteins in Microglia by Flow Cytometry","abstract":null,"journal":"Frontiers in Molecular Neuroscience","year":2020,"id":622698,"datarank":0.5101796072493234,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"self_citation_contribution":0.5101796072493234,"citation_network_contribution":0.0,"self_endowment_contribution":0.5101796072493234,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":29,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1609041,"name":"Paolo d’Errico","orcid":null,"position":1,"is_corresponding":false},{"id":1609042,"name":"Lukas S. Amann","orcid":null,"position":2,"is_corresponding":false},{"id":1609044,"name":"Hana Janova","orcid":null,"position":3,"is_corresponding":false},{"id":559596,"name":"Sonja M. Wojcik","orcid":"0009-0007-6106-252X","position":4,"is_corresponding":false},{"id":60935,"name":"Melanie Meyer-Luehmann","orcid":null,"position":5,"is_corresponding":false},{"id":1609048,"name":"Lawrence Rajendran","orcid":null,"position":6,"is_corresponding":false},{"id":894290,"name":"Peter Wieghofer","orcid":"0000-0003-4959-1182","position":7,"is_corresponding":false},{"id":1609049,"name":"Rosa C. Paolicelli","orcid":null,"position":8,"is_corresponding":false},{"id":549961,"name":"Knut Biber","orcid":"0000-0002-8815-1705","position":9,"is_corresponding":false},{"id":109969,"name":"Simone Brioschi","orcid":"0000-0002-3849-3926","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Detection of Synaptic Proteins in Microglia by Flow Cytometry","abstract":", thereby playing a key role in synaptic refinement and modulation of brain connectivity. This phenomenon was mainly investigated in immunofluorescence staining and confocal microscopy. However, a quantification of synaptic material in microglia using these techniques is extremely time-consuming and labor-intensive. To address this issue, we aimed to quantify synaptic proteins in microglia using flow cytometry. With this approach, we first showed that microglia from the healthy adult mouse brain contain a detectable level of VGLUT1 protein. Next, we found more than two-fold increased VGLUT1 immunoreactivity in microglia from the developing brain (P15) as compared to adult microglia. These data indicate that microglia-mediated synaptic pruning mostly occurs during the brain developmental period. We then quantified the VGLUT1 staining in microglia in two transgenic models characterized by pathological microglia-mediated synaptic pruning. In the 5xFAD mouse model of Alzheimer's disease (AD) microglia exhibited a significant increase in VGLUT1 immunoreactivity before the onset of amyloid pathology. Moreover, conditional deletion of TDP-43 in microglia, which causes a hyper-phagocytic phenotype associated with synaptic loss, also resulted in increased VGLUT1 immunoreactivity within microglia. This work provides a quantitative assessment of synaptic proteins in microglia, under homeostasis, and in mouse models of disease.","is_dataset_classified":null,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33132837","pmcid":"PMC7550663","openalex_id":"https://openalex.org/W3090355856","authors":[],"funders":[{"funder_name":"European Research Council","grant_id":"804949","title":"Targeting pathological synaptic pruning by microglia in neurodegeneration"}],"total_grants":1,"fwci":1.775,"citation_percentile":0.85688901,"influential_citations":0,"citation_trend":[{"year":2021,"count":3},{"year":2022,"count":8},{"year":2023,"count":10},{"year":2024,"count":3},{"year":2025,"count":3},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.3389/fnmol.2020.00149","host_type":"journal"},{"url":"https://doi.org/10.3389/fnmol.2020.00149","host_type":"publisher"},{"url":"https://www.frontiersin.org/article/10.3389/fnmol.2020.00149/full","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33132837","host_type":"repository"},{"url":"https://serval.unil.ch/notice/serval:BIB_49FE862930E6","host_type":"repository"},{"url":"https://iris.unil.ch/handle/iris/129955","host_type":"repository"},{"url":"https://doaj.org/article/4212dc9e7c4743df81cd46c466e44482","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7550663","host_type":"repository"},{"url":"http://hdl.handle.net/21.11116/0000-0007-4EA6-7","host_type":"repository"},{"url":"https://nbn-resolving.org/urn:nbn:de:bvb:384-opus4-897574","host_type":"repository"},{"url":"https://iris.unil.ch/bitstreams/7d2f7cf6-8067-4833-bf24-3b6c5cba15f8/download","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7550663","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7550663?pdf=render","host_type":"Europe_PMC"},{"url":"https://www.frontiersin.org/articles/10.3389/fnmol.2020.00149/pdf","host_type":""},{"url":"http://dx.doi.org/10.3389/fnmol.2020.00149","host_type":""},{"url":"https://dx.doi.org/10.3389/fnmol.2020.00149","host_type":""},{"url":"https://sonar.ch/global/documents/26038","host_type":""},{"url":"https://hdl.handle.net/21.11116/0000-0007-4EA6-7","host_type":""},{"url":"https://serval.unil.ch/resource/serval:BIB_49FE862930E6.P001/REF.pdf","host_type":""},{"url":"http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_49FE862930E68","host_type":""},{"url":"https://opus.bibliothek.uni-augsburg.de/opus4/files/89757/fnmol-13-00149.pdf","host_type":""},{"url":"https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/89757","host_type":""},{"url":"https://doi.org/https://doi.org/10.3389/fnmol.2020.00149","host_type":""}],"fields_of_study":["Neuroinflammation and Neurodegeneration Mechanisms","Alzheimer's disease research and treatments","Neuroscience and Neuropharmacology Research","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Microglia","Synaptic pruning","Neuroscience","Biology","Genetically modified mouse","Phagocytosis","Pathology","Cell biology","Transgene","Inflammation","Medicine","Immunology","Biochemistry","VGluT1","5Xfad Model","Tdp-43 Conditional Knock-Out","ddc:610","5xFAD model; TDP-43 conditional knock-out; VGLUT1; microglia; synaptic pruning","610","Neurosciences. 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