{"doi":"10.3389/fmicb.2023.1303387","title":"Escherichia coli O157:H7 tir 255 T > A allele strains differ in chromosomal and plasmid composition","abstract":"Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains with the T allele in the translocated intimin receptor polymorphism ( tir ) 255 A > T gene associate with human disease more than strains with an A allele; however, the allele is not thought to be the direct cause of this difference. We sequenced a diverse set of STEC O157:H7 strains (26% A allele, 74% T allele) to identify linked differences that might underlie disease association. The average chromosome and pO157 plasmid size and gene content were significantly greater within the tir 255 A allele strains. Eighteen coding sequences were unique to tir 255 A allele chromosomes, and three were unique to tir 255 T allele chromosomes. There also were non-pO157 plasmids that were unique to each tir 255 allele variant. The overall average number of prophages did not differ between tir 255 allele strains; however, there were different types between the strains. Genomic and mobile element variation linked to the tir 255 polymorphism may account for the increased frequency of the T allele isolates in human disease.","journal":"Frontiers in Microbiology","year":2023,"id":384092,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9318,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1151375,"name":"Michael L. Clawson","orcid":"0000-0002-3355-5390","position":1,"is_corresponding":false},{"id":1151376,"name":"Gregory P. Harhay","orcid":"0000-0002-0129-6822","position":2,"is_corresponding":false},{"id":415939,"name":"Mark Eppinger","orcid":"0000-0001-5267-5219","position":3,"is_corresponding":false},{"id":1151377,"name":"Dayna M. Harhay","orcid":"0000-0002-7203-8951","position":4,"is_corresponding":false},{"id":106877,"name":"Timothy P. L. Smith","orcid":"0000-0003-1611-6828","position":5,"is_corresponding":false},{"id":415938,"name":"James L. Bono","orcid":"0000-0001-5146-1171","position":6,"is_corresponding":false},{"id":1151374,"name":"Margaret D. Weinroth","orcid":"0000-0001-8351-395X","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-19T01:17:33.041385Z","pmid":"38169669","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}