{"doi":"10.3389/fmicb.2022.1088941","title":"5’UTR sequences influence protein levels in Escherichia coli by regulating translation initiation and mRNA stability","abstract":"A set of 41 synthetic 5’UTRs with different theoretical translation initiation rates were generated to explore the role of 5’UTRs in the regulation of protein levels in Escherichia coli. The roles of the synthetic 5’UTRs in regulating the expression of different reporter genes were analyzed in vivo. Protein levels varied substantially between the different constructs but for most of the 5’UTRs, protein levels were not correlated with theoretical translation initiation rates. Large variations in mRNA concentrations were measured with the different 5’UTRs even though the same concentration of transcription inducer was used in each case. 5’UTRs were also found to strongly affect mRNA stability, and these changes in mRNA stability often contributed to observed differences in mRNA concentration. Unexpectedly, the effect of the 5’UTRs on mRNA half-lives was found to vary depending on the downstream reporter gene. These results clearly demonstrate that 5’UTRs contribute to gene expression regulation at the level of translation initiation and of mRNA stability, to an extent that depends on the nature of the downstream gene.","journal":"Frontiers in 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Girbal","orcid":null,"position":2,"is_corresponding":false},{"id":174655,"name":"Sébastien Nouaille","orcid":null,"position":3,"is_corresponding":false},{"id":132914,"name":"Fan Chen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36620028","pmcid":"PMC9810816","openalex_id":"https://openalex.org/W4313210658","authors":[],"funders":[{"funder_name":"French National Research Agency (ANR)","grant_id":"ANR-18-CE43-0010","title":"Engineering RNA life cycle to optimize economy of microbial energy: application to the bioconversion of biomass-derived carbon sources"},{"funder_name":"French National Research Agency","grant_id":"","title":null},{"funder_name":"China Scholarship 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and protein synthesis mechanisms","Bacterial Genetics and Biotechnology","RNA Research and Splicing","Medicine","Biology","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Messenger RNA","Translation (biology)","Untranslated region","Translational regulation","Escherichia coli","Protein biosynthesis","Translational efficiency","Biology","Gene expression","Three prime untranslated region","Gene","Reporter gene","Five prime untranslated region","Cell biology","Eukaryotic translation","Regulation of gene expression","Molecular biology","Genetics","Gene expression regulation","Post-transcriptional regulation","Translation initiation","mRNA stability","Protein Level Regulation","Molecular Biology/Genomics [q-bio.GN]","[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry","612","[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology","Microbiology","QR1-502","[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular 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