{"doi":"10.3389/fmicb.2019.02900","title":"Diverse Expression of Antimicrobial Activities Against Bacterial Vaginosis and Urinary Tract Infection Pathogens by Cervicovaginal Microbiota Strains of Lactobacillus gasseri and Lactobacillus crispatus","abstract":null,"journal":"Frontiers in Microbiology","year":2019,"id":643541,"datarank":0.6414999178524083,"base_score":4.276666119016055,"endowment":4.276666119016055,"self_citation_contribution":0.6414999178524083,"citation_network_contribution":0.0,"self_endowment_contribution":0.6414999178524083,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":71,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1674483,"name":"Diane L. Pho Viet Ahn","orcid":null,"position":1,"is_corresponding":false},{"id":1674484,"name":"Vanessa Lievin-Le Moal","orcid":null,"position":2,"is_corresponding":false},{"id":1674482,"name":"Fabrice Atassi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Diverse Expression of Antimicrobial Activities Against Bacterial Vaginosis and Urinary Tract Infection Pathogens by Cervicovaginal Microbiota Strains of Lactobacillus gasseri and Lactobacillus crispatus","abstract":"Objectives: We aimed to analyze the strain-by-strain expression of a large panel of antimicrobial activities counteracting the virulence mechanisms of bacterial vaginosis-associated P. bivia and G. vaginalis, pyelonephritis-associated E. coli, and recurrent cystitis- and preterm labor-associated E. coli by L. gasseri and L. crispatus strains resident of the cervicovaginal microbiota of healthy women. Methods: We evaluated the antimicrobial properties of human cervicovaginal microbiota-associated L. gasseri and L. crispatus cells and secreted molecules, focusing on bactericidal and bacteriostatic effects on free, adhering, or internalized pathogens and biofilm disrupting activity; the inhibition of pathogen association with cervix epithelial cells by competition, exclusion, or displacement; and protective properties against the deleterious cellular effects of specific pathogen toxins. Results: We found that no antimicrobial activity is specific to resident L. gasseri or L. crispatus species. Non-strain specific antimicrobial activity was exerted by secreted lactic acid, which killed the microbial pathogens by direct contact. The Lactobacillus cells exhibited another non-strain specific activity which inhibited the association of microbial pathogens with cervix epithelial cells with varying efficiency, partially protecting the cells against lysis and detachment triggered by toxin-producing G. vaginalis and uropathogenic E. coli. Potent strain-specific bactericidal activity was exerted by a limited number of resident L. gasseri strains with the capacity to produce and secrete antibiotic-like compounds. These compounds eradicated the microbial pathogens pre-associated with the surface of cervix epithelial cells, providing efficient protection of the cells against the deleterious effects triggered by toxin-producing G. vaginalis and uropathogenic E. coli. Furthermore, these compounds crossed the cell membrane to kill the pre-internalized microbial pathogens. Conclusion: Our findings show that human microbiota-associated cervicovaginal strains of L. gasseri and L. crispatus share common lactic acid-associated antimicrobial activities. Our results also provide evidence of strain-level specificity for certain antimicrobial properties among cervicovaginal L. gasseri and L. crispatus strains, indicating that the presence of a particular species in the vaginal microbiota is not sufficient to determine its benefit to the host. A full repertory of strain-specific properties should be evaluated in choosing vaginal microbiota-associated Lactobacillus isolates for the development of live biotherapeutic strategies","is_dataset_classified":null,"base_score":4.276666119016055,"endowment":4.276666119016055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31921075","pmcid":"PMC6933176","openalex_id":"https://openalex.org/W2995296462","authors":[],"funders":[],"total_grants":0,"fwci":4.5515,"citation_percentile":0.95541047,"influential_citations":0,"citation_trend":[{"year":2020,"count":9},{"year":2021,"count":11},{"year":2022,"count":11},{"year":2023,"count":17},{"year":2024,"count":10},{"year":2025,"count":7},{"year":2026,"count":6}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.frontiersin.org/articles/10.3389/fmicb.2019.02900/pdf","host_type":"journal"},{"url":"https://www.frontiersin.org/articles/10.3389/fmicb.2019.02900/pdf","host_type":"publisher"},{"url":"https://www.frontiersin.org/article/10.3389/fmicb.2019.02900/full","host_type":"publisher"},{"url":"https://doi.org/10.3389/fmicb.2019.02900","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31921075","host_type":"repository"},{"url":"https://hal.sorbonne-universite.fr/hal-03981163","host_type":"repository"},{"url":"https://doaj.org/article/938529d37d284a82a8b7457ffab8779c","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6933176","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6933176","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6933176?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Urinary Tract Infections Management","Reproductive tract infections research","Gut microbiota and health"],"mesh_terms":[],"keywords":["Lactobacillus gasseri","Microbiology","Bacterial vaginosis","Lactobacillus crispatus","Biology","Antimicrobial","Pathogen","Lactobacillus","Biofilm","Virulence","Bacteria","Urinary tract infections","Cervicovaginal Microbiota"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Gender equality"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-08T17:36:39.816363Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}