{"doi":"10.3389/fmed.2020.00112","title":"Single-Cell Transcriptional Profiling of Cells Derived From Regenerating Alveolar Ducts","abstract":"Lung regeneration occurs in a variety of adult mammals after surgical removal of one lung (pneumonectomy). Previous studies of murine post-pneumonectomy lung growth have identified regenerative \"hotspots\" in subpleural alveolar ducts; however, the cell-types participating in this process remain unclear. To identify the single cells participating in post-pneumonectomy lung growth, we used laser microdissection, enzymatic digestion and microfluidic isolation. Single-cell transcriptional analysis of the murine alveolar duct cells was performed using the C1 integrated fluidic circuit (Fluidigm) and a custom PCR panel designed for lung growth and repair genes. The multi-dimensional data set was analyzed using visualization software based on the tSNE algorithm. The analysis identified 6 cell clusters; 1 cell cluster was present only after pneumonectomy. This post-pneumonectomy cluster was significantly less transcriptionally active than 3 other clusters and may represent a transitional cell population. A provisional cluster identity for 4 of the 6 cell clusters was obtained by embedding bulk transcriptional data into the tSNE analysis. The transcriptional pattern of the 6 clusters was further analyzed for genes associated with lung repair, matrix production, and angiogenesis. The data demonstrated that multiple cell-types (clusters) transcribed genes linked to these basic functions. We conclude that the coordinated gene expression across multiple cell clusters is likely a response to a shared regenerative microenvironment within the subpleural alveolar ducts.","journal":"Frontiers in Medicine","year":2020,"id":111012,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9505,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":527275,"name":"Robert D. Bennett","orcid":"0000-0002-1336-970X","position":1,"is_corresponding":false},{"id":373739,"name":"Willi L. Wagner","orcid":"0000-0002-2748-5429","position":2,"is_corresponding":false},{"id":527276,"name":"Cristian D. Valenzuela","orcid":"0000-0002-8694-9174","position":3,"is_corresponding":false},{"id":527277,"name":"Andrew B. Servais","orcid":"0000-0001-8656-9166","position":4,"is_corresponding":false},{"id":527278,"name":"Akira Tsuda","orcid":"0000-0003-4542-1573","position":5,"is_corresponding":false},{"id":528144,"name":"Saumyadipta Pyne","orcid":null,"position":6,"is_corresponding":false},{"id":490132,"name":"Shuqiang Li","orcid":"0000-0002-9916-7702","position":7,"is_corresponding":false},{"id":527279,"name":"Jonna Grimsby","orcid":"0000-0003-2304-7656","position":8,"is_corresponding":false},{"id":527280,"name":"Prapti Pokharel","orcid":"0009-0009-6316-5527","position":9,"is_corresponding":false},{"id":12216,"name":"Kenneth J. Livak","orcid":"0000-0001-9105-5856","position":10,"is_corresponding":false},{"id":373742,"name":"Maximilian Ackermann","orcid":"0000-0001-9996-2477","position":11,"is_corresponding":false},{"id":89096,"name":"Paul C. Blainey","orcid":"0000-0002-4889-8783","position":12,"is_corresponding":false},{"id":373747,"name":"Steven J. Mentzer","orcid":"0000-0003-1496-6044","position":13,"is_corresponding":false},{"id":228729,"name":"Alexandra B. Ysasi","orcid":"0000-0002-2932-870X","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-18T23:13:01.914939Z","pmid":"32373614","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}