{"doi":"10.3389/fmed.2019.00331","title":"Non-ionizing 405 nm Light as a Potential Bactericidal Technology for Platelet Safety: Evaluation of in vitro Bacterial Inactivation and in vivo Platelet Recovery in Severe Combined Immunodeficient Mice","abstract":"Bacterial contamination of ex vivo stored platelets is a cause of transfusion-transmitted infection. Violet-blue 405 nm light has recently demonstrated efficacy in reducing the bacterial burden in blood plasma, and its operational benefits such as non-ionizing nature, penetrability, and non-requirement for photosensitizing agents, provide a unique opportunity to develop this treatment for in situ treatment of ex vivo stored platelets as a tool for bacterial reduction. Sealed bags of platelet concentrates, seeded with low-level Staphylococcus aureus contamination, were 405 nm light-treated (3-10 mWcm-2) up to 8-hr. Antimicrobial efficacy and dose efficiency was evaluated by quantification of the post-treatment surviving bacterial contamination levels. Platelets treated with 10 mWcm-2 for 8-hr were further evaluated for survival and recovery in severe combined immunodeficient (SCID) mice. Significant inactivation of bacteria in platelet concentrates was achieved using all irradiance levels, with 99.6-100% inactivation achieved by 8-hr (P0.05). This report paves the way for further comprehensive studies to test 405nm light treatment as a bactericidal technology for stored platelets.","journal":"Frontiers in Medicine","year":2020,"id":68142,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":361172,"name":"Monique P. Gelderman","orcid":"0000-0002-6543-6986","position":1,"is_corresponding":false},{"id":361173,"name":"Sandhya Kulkarni","orcid":"0000-0002-1411-192X","position":2,"is_corresponding":false},{"id":361174,"name":"Rachael Tomb","orcid":"0000-0003-3261-7673","position":3,"is_corresponding":false},{"id":361175,"name":"Caitlin Stewart","orcid":"0000-0001-7645-7216","position":4,"is_corresponding":false},{"id":361176,"name":"J.G. Anderson","orcid":"0000-0003-4151-1619","position":5,"is_corresponding":false},{"id":361177,"name":"S.J. MacGregor","orcid":"0000-0002-0808-585X","position":6,"is_corresponding":false},{"id":361178,"name":"Chintamani D. Atreya","orcid":"0000-0001-7393-2157","position":7,"is_corresponding":false},{"id":362767,"name":"Michelle Maclean","orcid":null,"position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-18T21:40:52.680475Z","pmid":"32010702","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}