{"doi":"10.3389/fmed.2018.00089","title":"Free-Floating Mesothelial Cells in Pleural Fluid After Lung Surgery","abstract":null,"journal":"Frontiers in Medicine","year":2018,"id":680659,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":527277,"name":"Andrew B. Servais","orcid":"0000-0001-8656-9166","position":1,"is_corresponding":false},{"id":228729,"name":"Alexandra B. Ysasi","orcid":"0000-0002-2932-870X","position":2,"is_corresponding":false},{"id":898674,"name":"Barry C. Gibney","orcid":"0000-0003-0594-7669","position":3,"is_corresponding":false},{"id":527276,"name":"Cristian D. Valenzuela","orcid":"0000-0002-8694-9174","position":4,"is_corresponding":false},{"id":373739,"name":"Willi L. Wagner","orcid":"0000-0002-2748-5429","position":5,"is_corresponding":false},{"id":373742,"name":"Maximilian Ackermann","orcid":"0000-0001-9996-2477","position":6,"is_corresponding":false},{"id":373747,"name":"Steven J. Mentzer","orcid":"0000-0003-1496-6044","position":7,"is_corresponding":false},{"id":1778382,"name":"Arne Kienzle","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Free-Floating Mesothelial Cells in Pleural Fluid After Lung Surgery","abstract":"OBJECTIVES: The mesothelium, the surface layer of the heart, lung, bowel, liver, and tunica vaginalis, is a complex tissue implicated in organ-specific diseases and regenerative biology; however, the mechanism of mesothelial repair after surgical injury is unknown. Previous observations indicated seeding of denuded mesothelium by free-floating mesothelial cells may contribute to mesothelial healing. In this study, we investigated the prevalence of mesothelial cells in pleural fluid during the 7 days following pulmonary surgery. STUDY DESIGN: Flow cytometry was employed to study pleural fluid of 45 patients after lung resection or transplantation. We used histologically validated mesothelial markers (CD71 and WT1) to estimate the prevalence of mesothelial cells. RESULTS: The viability of pleural fluid cells approached 100%. Leukocytes and mesothelial cells were identified in the pleural fluid within the first week after surgery. The leukocyte concentration was relatively stable at all time points. In contrast, mesothelial cells, identified by CD71 and WT1 peaked on POD3. The broad expression of CD71 molecule in postoperative pleural fluid suggests that many of the free-floating non-leukocyte cells were activated or proliferative mesothelial cells. CONCLUSION: We demonstrated that pleural fluid post lung surgery is a source of mesothelial cells; most of these cells appear to be viable and, as shown by CD71 staining, activated mesothelial cells. The observed peak of mesothelial cells on POD3 is consistent with a potential reparative role of free-floating mesothelial cells after pulmonary surgery.","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29675416","pmcid":"PMC5895720","openalex_id":"https://openalex.org/W2796187549","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"HL94567, HL134229, HL007734, CA009535 and ES000002","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB1066","title":null},{"funder_name":"NCI NIH HHS","grant_id":"T32 CA009535","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL094567","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"T32 HL007734","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"F32 HL134229","title":null},{"funder_name":"NIEHS NIH HHS","grant_id":"P30 ES000002","title":null},{"funder_name":"National Institutes of Health","grant_id":"7T32CA009535-01","title":"ADVANCED TRAINING IN SURGICAL ONCOLOGY"},{"funder_name":"National Institutes of Health","grant_id":"5R01HL094567-04","title":"Adult Tissue Morphogenesis: Functinoal Regulation of Intussusceptive Antiogenesis"},{"funder_name":"National Institutes of Health","grant_id":"2T32HL007734-11","title":"Harvard-Longwood Research Training in Vascular Surgery"},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"National Institutes of Health","grant_id":"1F32HL134229-01","title":"Formation of the Elastin Line Element in the Developing Lung"},{"funder_name":"Boehringer Ingelheim","grant_id":"","title":null}],"total_grants":13,"fwci":1.9139,"citation_percentile":0.86177521,"influential_citations":0,"citation_trend":[{"year":2020,"count":7},{"year":2021,"count":5},{"year":2022,"count":1},{"year":2024,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.frontiersin.org/articles/10.3389/fmed.2018.00089/pdf","host_type":"journal"},{"url":"https://www.frontiersin.org/articles/10.3389/fmed.2018.00089/pdf","host_type":"publisher"},{"url":"http://journal.frontiersin.org/article/10.3389/fmed.2018.00089/full","host_type":"publisher"},{"url":"https://doi.org/10.3389/fmed.2018.00089","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29675416","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5895720","host_type":"repository"},{"url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:37067873","host_type":"repository"},{"url":"https://doaj.org/article/4b2c63c3432c40bbacb0d12e37f78ca0","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5895720","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5895720?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.3389/fmed.2018.00089","host_type":""},{"url":"https://dx.doi.org/10.3389/fmed.2018.00089","host_type":""},{"url":"https://doi.org/https://doi.org/10.3389/fmed.2018.00089","host_type":""}],"fields_of_study":["Pleural and Pulmonary Diseases","Tissue Engineering and Regenerative Medicine","Occupational and environmental lung diseases","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Mesothelial Cell","Mesothelium","Pathology","Medicine","Lung","Internal medicine","Pneumonectomy","Mesothelial Cells","Pleural Fluid","Lung Regeneration","Lung Healing","Medicine (General)","610","R5-920","617"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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