{"doi":"10.3389/fimmu.2025.1539773","title":"Decoding plasma cell maturation dynamics with BCMA","abstract":"<jats:p>Plasma cells provide protective antibodies following an infection or vaccination. A network of intrinsic and extrinsic factors fine-tunes the generation of a heterogenous plasma cell pool with varying metabolic requirements, transcriptional profiles and lifespans. Among these, the B cell maturation antigen (BCMA) has been implicated in the APRIL-mediated survival of long-lived plasma cells. To characterize the terminal maturation of plasma cells, we constructed a BCMA reporter mouse (BCMA:Tom) that exclusively labeled antibody-secreting cells and revealed that BCMA:Tom expression varied by IgH isotype and increased with plasma cell maturity. The BCMA reporter, used alongside the Blimp1-GFP reporter, also allowed detailed tracking of plasma cell development and highlighted the importance of the <jats:italic>in vivo</jats:italic> microenvironment to complete plasma cell maturation. Therefore, the BCMA:Tom reporter mouse provides a valuable tool for tracking plasma cell development and maturation with flow cytometry or advanced imaging techniques, enabling a deeper understanding of the mechanisms regulating plasma cell heterogeneity and longevity.</jats:p>","journal":"Frontiers in Immunology","year":2025,"id":611844,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":1,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1575041,"name":"Shannon R. 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Among these, the B cell maturation antigen (BCMA) has been implicated in the APRIL-mediated survival of long-lived plasma cells. To characterize the terminal maturation of plasma cells, we constructed a BCMA reporter mouse (BCMA:Tom) that exclusively labeled antibody-secreting cells and revealed that BCMA:Tom expression varied by IgH isotype and increased with plasma cell maturity. The BCMA reporter, used alongside the Blimp1-GFP reporter, also allowed detailed tracking of plasma cell development and highlighted the importance of the <jats:italic>in vivo</jats:italic> microenvironment to complete plasma cell maturation. Therefore, the BCMA:Tom reporter mouse provides a valuable tool for tracking plasma cell development and maturation with flow cytometry or advanced imaging techniques, enabling a deeper understanding of the mechanisms regulating plasma cell heterogeneity and longevity.</jats:p>","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":1,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40134424","pmcid":"PMC11932843","openalex_id":"https://openalex.org/W4408315884","authors":[],"funders":[],"total_grants":0,"fwci":3.9149,"citation_percentile":0.9406979,"influential_citations":0,"citation_trend":[{"year":2025,"count":4},{"year":2026,"count":6}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1539773/pdf","host_type":"journal"},{"url":"https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1539773/pdf","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fimmu.2025.1539773/full","host_type":"publisher"},{"url":"https://doi.org/10.3389/fimmu.2025.1539773","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40134424","host_type":"repository"},{"url":"https://open.fau.de/handle/openfau/35546","host_type":"repository"},{"url":"https://doaj.org/article/15cbe8df6e8b4f33b713165329846efe","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11932843","host_type":"repository"},{"url":"https://open.fau.de/bitstreams/a7397902-23cf-491e-99c2-c552fdba5915/download","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11932843","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11932843?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["T-cell and B-cell Immunology","Immune Cell Function and Interaction","Complement system in diseases","Animals","Plasma Cells","B-Cell Maturation Antigen","Mice","Cell Differentiation","Mice, Transgenic","Genes, Reporter","Positive Regulatory Domain I-Binding Factor 1","Mice, Inbred C57BL"],"mesh_terms":["Positive Regulatory Domain I-Binding Factor 1","Animals","Cell Differentiation","Mice, Inbred C57BL","Mice, Transgenic","Plasma Cells","Genes, Reporter","Mice","B-Cell Maturation Antigen"],"keywords":["Plasma cell","Cell biology","Reporter gene","Flow cytometry","Biology","Reprogramming","Antibody","Cell","Immunology","Gene expression","Gene","Genetics","Spleen","Bone marrow","Survival","Thymus","Plasma cells","Bcma (Tnfrsf17)","Antibody-Secreting Cells (Asc)"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[{"doi":"10.17169/refubium-47306","title":"Decoding plasma cell maturation dynamics with BCMA","publisher":"Freie Universität Berlin","resource_type":"Text"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-01T23:14:19.619844Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}