{"doi":"10.3389/fimmu.2023.1021824","title":"IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium","abstract":"Mucosal delivery of IL-27 has been shown to have a therapeutic benefit in murine models of inflammatory bowel disease (IBD). The IL-27 effect was associated with phosphorylated STAT1 (pSTAT1), a product of IL27 receptor signaling, in bowel tissue. To determine whether IL-27 acted directly on colonic epithelium, murine colonoids and primary intact colonic crypts were shown to be unresponsive to IL-27 in vitro and to lack detectable IL-27 receptors. On the other hand, macrophages, which are present in inflamed colon tissue, were responsive to IL-27 in vitro . IL-27 induced pSTAT1 in macrophages, the transcriptome indicated an IFN-like signature, and supernatants induced pSTAT1 in colonoids. IL-27 induced anti-viral activity in macrophages and MHC Class II induction. We conclude that the effects of mucosal delivery of IL-27 in murine IBD are in part based on the known effects of IL27 inducing immunosuppression of T cells mediated by IL-10. We also conclude that IL-27 has potent effects on macrophages in inflamed colon tissue, generating mediators that in turn act on colonic epithelium.","journal":"Frontiers in Immunology","year":2023,"id":351058,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1095777,"name":"Mairi H McLean","orcid":"0000-0002-7443-2653","position":1,"is_corresponding":false},{"id":771778,"name":"Julie A. Hixon","orcid":"0000-0002-6505-2392","position":2,"is_corresponding":false},{"id":583570,"name":"Sergio M. Pontejo","orcid":"0000-0002-7756-9831","position":3,"is_corresponding":false},{"id":690665,"name":"Tregei Starr","orcid":"0000-0003-1561-8834","position":4,"is_corresponding":false},{"id":420497,"name":"Courtney S. Malo","orcid":"0000-0001-9674-3826","position":5,"is_corresponding":false},{"id":349715,"name":"Margaret C. Cam","orcid":null,"position":6,"is_corresponding":false},{"id":428819,"name":"Lisa A. Ridnour","orcid":"0000-0001-9446-8349","position":7,"is_corresponding":false},{"id":245858,"name":"Heather D. Hickman","orcid":"0000-0003-4168-4894","position":8,"is_corresponding":false},{"id":644605,"name":"Olivia Steele‐Mortimer","orcid":"0000-0002-3744-4217","position":9,"is_corresponding":false},{"id":228148,"name":"David A. Wink","orcid":"0000-0002-5652-7480","position":10,"is_corresponding":false},{"id":309027,"name":"Howard A. Young","orcid":"0000-0002-3118-5111","position":11,"is_corresponding":false},{"id":228149,"name":"Daniel W. McVicar","orcid":"0000-0002-1112-5111","position":12,"is_corresponding":false},{"id":257719,"name":"Wenqing Li","orcid":"0000-0002-8336-5552","position":13,"is_corresponding":false},{"id":272369,"name":"Scott K. Durum","orcid":"0000-0003-1307-9582","position":14,"is_corresponding":false},{"id":393439,"name":"Caroline Andrews","orcid":"0000-0001-5818-4365","position":0,"is_corresponding":true}],"reference_count":23,"raw_metadata":null,"created_at":"2026-07-19T01:12:41.740237Z","pmid":"37153622","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}