{"doi":"10.3389/fimmu.2022.908398","title":"Plasmablast Expansion Following the Tetravalent, Live-Attenuated Dengue Vaccine Butantan-DV in DENV-Naïve and DENV-Exposed Individuals in a Brazilian Cohort","abstract":"An effective vaccine against the dengue virus (DENV) should induce a balanced, long-lasting antibody (Ab) response against all four viral serotypes. The burst of plasmablasts in the peripheral blood after vaccination may reflect enriched vaccine-specific Ab secreting cells. Here we characterize the acute plasmablast responses from naïve and DENV-exposed individuals following immunization with the live attenuated tetravalent (LAT) Butantan DENV vaccine (Butantan-DV). The frequency of circulating plasmablasts was determined by flow cytometric analysis of fresh whole blood specimens collected from 40 participants enrolled in the Phase II Butantan-DV clinical trial (NCT01696422) before and after (days 6, 12, 15 and 22) vaccination. We observed a peak in the number of circulating plasmablast at day 15 after vaccination in both the DENV naïve and the DENV-exposed vaccinees. DENV-exposed vaccinees experienced a significantly higher plasmablast expansion. In the DENV-naïve vaccinees, plasmablasts persisted for approximately three weeks longer than among DENV-exposed volunteers. Our findings indicate that the Butantan-DV can induce plasmablast responses in both DENV-naïve and DENV-exposed individuals and demonstrate the influence of pre-existing DENV immunity on Butantan DV-induced B-cell responses.","journal":"Frontiers in Immunology","year":2022,"id":290167,"datarank":0.3114160567727907,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.07000036990767561,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.07000036990767561,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":4,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9609,"is_data_producer":true,"deposit_databanks":{"ClinicalTrials.gov":["NCT01696422"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":527093,"name":"Diogo M. Magnani","orcid":"0000-0002-1773-2406","position":1,"is_corresponding":false},{"id":891735,"name":"Priscilla R. Costa","orcid":"0000-0001-6812-4999","position":2,"is_corresponding":false},{"id":817784,"name":"Vivian Iida Avelino‐Silva","orcid":"0000-0002-6660-3088","position":3,"is_corresponding":false},{"id":432420,"name":"Michael J. Ricciardi","orcid":"0000-0002-8098-8666","position":4,"is_corresponding":false},{"id":972176,"name":"María do Carmo Sampaio Tavares Timenetsky","orcid":"0000-0001-8317-0539","position":5,"is_corresponding":false},{"id":972575,"name":"Raphaella Goulart","orcid":null,"position":6,"is_corresponding":false},{"id":817783,"name":"Carolina Argondizo Correia","orcid":"0000-0003-4526-0896","position":7,"is_corresponding":false},{"id":891736,"name":"Mariana Prado Marmorato","orcid":"0000-0002-0644-0862","position":8,"is_corresponding":false},{"id":891742,"name":"Lílian Pereira Ferrari","orcid":"0000-0001-8680-8200","position":9,"is_corresponding":false},{"id":972576,"name":"Zelinda B. Nakagawa","orcid":null,"position":10,"is_corresponding":false},{"id":972577,"name":"Claudia Satiko Tomiyama","orcid":null,"position":11,"is_corresponding":false},{"id":972578,"name":"Helena Tomiyama","orcid":null,"position":12,"is_corresponding":false},{"id":482397,"name":"Jorge Kalil","orcid":"0000-0001-8415-4274","position":13,"is_corresponding":false},{"id":571566,"name":"Ricardo Palácios","orcid":"0000-0002-1410-8579","position":14,"is_corresponding":false},{"id":972177,"name":"Alexander Roberto Precioso","orcid":"0000-0001-8657-9292","position":15,"is_corresponding":false},{"id":279513,"name":"David I. Watkins","orcid":"0000-0001-6582-3305","position":16,"is_corresponding":false},{"id":285114,"name":"Esper G. Kallás","orcid":"0000-0003-2026-6925","position":17,"is_corresponding":false},{"id":333939,"name":"Cássia Gisele Terrassani Silveira","orcid":"0000-0002-0932-7769","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T00:30:26.667578Z","pmid":"35837409","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}