{"doi":"10.3389/fimmu.2021.804932","title":"Identification and Tracking of Alloreactive T Cell Clones in Rhesus Macaques Through the RM-scTCR-Seq Platform","abstract":"T cell receptor (TCR) clonotype tracking is a powerful tool for interrogating T cell mediated immune processes. New methods to pair a single cell’s transcriptional program with its TCR identity allow monitoring of T cell clonotype-specific transcriptional dynamics. While these technologies have been available for human and mouse T cells studies, they have not been developed for Rhesus Macaques (RM), a critical translational organism for autoimmune diseases, vaccine development and transplantation. We describe a new pipeline, ‘RM-scTCR-Seq’, which, for the first time, enables RM specific single cell TCR amplification, reconstruction and pairing of RM TCR’s with their transcriptional profiles. We apply this method to a RM model of GVHD, and identify and track in vitro detected alloreactive clonotypes in GVHD target organs and explore their GVHD driven cytotoxic T cell signature. This novel, state-of-the-art platform fundamentally advances the utility of RM to study protective and pathogenic T cell responses.","journal":"Frontiers in Immunology","year":2022,"id":274944,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":944561,"name":"Ryan A. Fleming","orcid":null,"position":1,"is_corresponding":false},{"id":615916,"name":"James J. Kaminski","orcid":"0000-0002-5137-7516","position":2,"is_corresponding":false},{"id":320162,"name":"Connor McGuckin","orcid":"0000-0001-8860-9926","position":3,"is_corresponding":false},{"id":943987,"name":"Xianliang Rui","orcid":"0009-0007-1219-4382","position":4,"is_corresponding":false},{"id":943988,"name":"Jennifer Lane","orcid":"0000-0001-8130-0176","position":5,"is_corresponding":false},{"id":943989,"name":"Paula Keskula","orcid":"0000-0002-0458-9153","position":6,"is_corresponding":false},{"id":944562,"name":"Lorenzo Cagnin","orcid":null,"position":7,"is_corresponding":false},{"id":1413,"name":"Alex K. Shalek","orcid":"0000-0001-5670-8778","position":8,"is_corresponding":false},{"id":104343,"name":"Victor Tkachev","orcid":"0000-0001-6011-0576","position":9,"is_corresponding":false},{"id":104356,"name":"Leslie S. Kean","orcid":"0000-0003-0475-3926","position":10,"is_corresponding":false},{"id":676999,"name":"Ulrike Gerdemann","orcid":"0000-0002-3600-0366","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T00:28:16.928746Z","pmid":"35154078","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}