{"doi":"10.3389/fimmu.2021.772864","title":"Chimeric Fusion (F) and Attachment (G) Glycoprotein Antigen Delivery by mRNA as a Candidate Nipah Vaccine","abstract":"Nipah virus (NiV) represents a significant pandemic threat with zoonotic transmission from bats-to-humans with almost annual regional outbreaks characterized by documented human-to-human transmission and high fatality rates. Currently, no vaccine against NiV has been approved. Structure-based design and protein engineering principles were applied to stabilize the fusion (F) protein in its prefusion trimeric conformation (pre-F) to improve expression and increase immunogenicity. We covalently linked the stabilized pre-F through trimerization domains at the C-terminus to three attachment protein (G) monomers, forming a chimeric design. These studies detailed here focus on mRNA delivery of NiV immunogens in mice, assessment of mRNA immunogen-specific design elements and their effects on humoral and cellular immunogenicity. The pre-F/G chimera elicited a strong neutralizing antibody response and a superior NiV-specific Tfh and other effector T cell response compared to G alone across both the mRNA and protein platforms. These findings enabled final candidate selection of pre-F/G Fd for clinical development.","journal":"Frontiers in Immunology","year":2021,"id":153461,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9592,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":105574,"name":"Anthony DiPiazza","orcid":"0000-0003-1989-8351","position":1,"is_corresponding":false},{"id":627251,"name":"Samantha Falcone","orcid":null,"position":2,"is_corresponding":false},{"id":105610,"name":"Tracy J. Ruckwardt","orcid":"0000-0002-0007-7169","position":3,"is_corresponding":false},{"id":105613,"name":"Kaitlyn M. Morabito","orcid":"0000-0002-4849-4690","position":4,"is_corresponding":false},{"id":87310,"name":"Olubukola M. Abiona","orcid":"0000-0002-2745-1691","position":5,"is_corresponding":false},{"id":105591,"name":"Lauren A. Chang","orcid":"0000-0002-3287-3987","position":6,"is_corresponding":false},{"id":291919,"name":"Ria T. Caringal","orcid":"0000-0001-8818-6708","position":7,"is_corresponding":false},{"id":105596,"name":"Vladimir Presnyak","orcid":"0000-0001-6695-7102","position":8,"is_corresponding":false},{"id":105594,"name":"Elisabeth Narayanan","orcid":"0000-0001-8836-181X","position":9,"is_corresponding":false},{"id":229148,"name":"Yaroslav Tsybovsky","orcid":"0000-0002-2159-5248","position":10,"is_corresponding":false},{"id":652370,"name":"Deepika Nair","orcid":null,"position":11,"is_corresponding":false},{"id":105580,"name":"Geoffrey B. Hutchinson","orcid":"0000-0003-0727-1682","position":12,"is_corresponding":false},{"id":266885,"name":"Guillaume B. E. Stewart-Jones","orcid":"0000-0002-2440-3790","position":13,"is_corresponding":false},{"id":291921,"name":"Lisa A. Kueltzo","orcid":"0000-0001-7157-320X","position":14,"is_corresponding":false},{"id":105572,"name":"Sunny Himansu","orcid":"0000-0002-7954-9674","position":15,"is_corresponding":false},{"id":105614,"name":"John R. Mascola","orcid":"0000-0002-5293-4695","position":16,"is_corresponding":false},{"id":105615,"name":"Andrea Carfı́","orcid":"0000-0001-5508-3809","position":17,"is_corresponding":false},{"id":87311,"name":"Barney S. Graham","orcid":"0000-0001-8112-0853","position":18,"is_corresponding":false},{"id":291918,"name":"Rebecca J. Loomis","orcid":"0000-0001-5578-4728","position":0,"is_corresponding":true}],"reference_count":103,"raw_metadata":null,"created_at":"2026-07-18T23:43:44.759273Z","pmid":"34956199","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}