{"doi":"10.3389/fimmu.2021.658372","title":"ARTEMIS: A Novel Mass-Spec Platform for HLA-Restricted Self and Disease-Associated Peptide Discovery","abstract":"Conventional immunoprecipitation/mass spectroscopy identification of HLA-restricted peptides remains the purview of specializing laboratories, due to the complexity of the methodology, and requires computational post-analysis to assign peptides to individual alleles when using pan-HLA antibodies. We have addressed these limitations with ARTEMIS: a simple, robust, and flexible platform for peptide discovery across ligandomes, optionally including specific proteins-of-interest, that combines novel, secreted HLA-I discovery reagents spanning multiple alleles, optimized lentiviral transduction, and streamlined affinity-tag purification to improve upon conventional methods. This platform fills a middle ground between existing techniques: sensitive and adaptable, but easy and affordable enough to be widely employed by general laboratories. We used ARTEMIS to catalog allele-specific ligandomes from HEK293 cells for seven classical HLA alleles and compared results across replicates, against computational predictions, and against high-quality conventional datasets. We also applied ARTEMIS to identify potentially useful, novel HLA-restricted peptide targets from oncovirus oncoproteins and tumor-associated antigens.","journal":"Frontiers in Immunology","year":2021,"id":177274,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9558,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":720935,"name":"Mi‐Youn Brusniak","orcid":"0000-0003-3950-1614","position":1,"is_corresponding":false},{"id":315176,"name":"Lisa Jones","orcid":"0000-0002-5122-8334","position":2,"is_corresponding":false},{"id":55140,"name":"Chenwei Lin","orcid":"0000-0001-7025-2770","position":3,"is_corresponding":false},{"id":302714,"name":"Andrew Fioré-Gartland","orcid":"0000-0001-7627-2166","position":4,"is_corresponding":false},{"id":721499,"name":"Chance Brock","orcid":null,"position":5,"is_corresponding":false},{"id":720936,"name":"Philip R. Gafken","orcid":"0000-0001-6092-4828","position":6,"is_corresponding":false},{"id":475528,"name":"Roland K. Strong","orcid":"0000-0002-1338-2189","position":7,"is_corresponding":false},{"id":476316,"name":"Kathryn A. K. Finton","orcid":null,"position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-18T23:47:28.018172Z","pmid":"33986749","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}