{"doi":"10.3389/fimmu.2021.610456","title":"Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children","abstract":"Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in healthy adults. In newborns, it can occasionally lead to a spectrum of malformations, the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues, we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to the ZIKV during pregnancy in the 2016–2017 Zika outbreak, who gave birth to infants affected by neurological complications or asymptomatic ones. Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools (CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed an increased CD4 and CD8 T-cell responses in mothers compared to children. The degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers, and children, while in CD8 T-cells, low responses were detected in these study groups. The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4 ZIKV MP. Conclusion: Donors with a history of ZIKV infection demonstrated long-term CD4 T cell immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities of CD8 T cells are markedly demonstrated in the early stages of infection, but less detected in the disease resolution phase, when the virus has already been eliminated. The responses of mothers' T cells to ZIKV MPs do not appear to be related to their children's clinical outcome. There was also no marked difference in the T cell responses to ZIKV MP between children affected or not with CZS. These data still need to be investigated, including the evaluation of the response of CD8 T cells to other ZIKV peptides.","journal":"Frontiers in Immunology","year":2021,"id":191669,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9578,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":757430,"name":"Fabiana Rabe Carvalho","orcid":"0000-0002-4713-233X","position":1,"is_corresponding":false},{"id":757431,"name":"Iury Amâncio Paiva","orcid":"0000-0002-3243-0414","position":2,"is_corresponding":false},{"id":757432,"name":"Débora Familiar-Macedo","orcid":"0000-0002-9229-5912","position":3,"is_corresponding":false},{"id":757433,"name":"Helver Gonçalves Dias","orcid":"0000-0001-5181-7078","position":4,"is_corresponding":false},{"id":615072,"name":"Alex Pauvolid‐Corrêa","orcid":"0000-0002-6924-157X","position":5,"is_corresponding":false},{"id":757434,"name":"Caroline Fernandes-Santos","orcid":"0000-0002-4984-1287","position":6,"is_corresponding":false},{"id":757435,"name":"Monique da Rocha Queiroz Lima","orcid":"0000-0001-9630-8736","position":7,"is_corresponding":false},{"id":448882,"name":"Mariana Gandini","orcid":"0000-0003-3603-6921","position":8,"is_corresponding":false},{"id":353736,"name":"Andréa Alice Silva","orcid":"0000-0001-5856-6128","position":9,"is_corresponding":false},{"id":757436,"name":"Silvia Maria Baêta Cavalcanti","orcid":"0000-0002-2994-7172","position":10,"is_corresponding":false},{"id":757437,"name":"Solange Artimos de Oliveira","orcid":"0000-0002-1862-2348","position":11,"is_corresponding":false},{"id":757438,"name":"Renata Artimos de Oliveira Vianna","orcid":"0000-0002-6416-7278","position":12,"is_corresponding":false},{"id":757439,"name":"Elzinandes Leal de Azeredo","orcid":"0000-0002-4119-0176","position":13,"is_corresponding":false},{"id":757440,"name":"Claudete Aparecida Araújo Cardoso","orcid":"0000-0002-7638-6814","position":14,"is_corresponding":false},{"id":292649,"name":"Alba Grifoni","orcid":"0000-0002-2209-5966","position":15,"is_corresponding":false},{"id":55670,"name":"Alessandro Sette","orcid":"0000-0001-7013-2250","position":16,"is_corresponding":false},{"id":103766,"name":"Daniela Weiskopf","orcid":"0000-0003-2968-7371","position":17,"is_corresponding":false},{"id":758006,"name":"Luzia Maria de‐Oliveira‐Pinto","orcid":null,"position":18,"is_corresponding":false},{"id":758005,"name":"Jéssica Badolato-Corrêa","orcid":null,"position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-18T23:49:35.285023Z","pmid":"33679748","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}