{"doi":"10.3389/fimmu.2020.614509","title":"Interleukin 10 Restores Lipopolysaccharide-Induced Alterations in Synaptic Plasticity Probed by Repetitive Magnetic Stimulation","abstract":"Systemic inflammation is associated with alterations in complex brain functions such as learning and memory. However, diagnostic approaches to functionally assess and quantify inflammation-associated alterations in synaptic plasticity are not well-established. In previous work, we demonstrated that bacterial lipopolysaccharide (LPS)-induced systemic inflammation alters the ability of hippocampal neurons to express synaptic plasticity, i.e., the long-term potentiation (LTP) of excitatory neurotransmission. Here, we tested whether synaptic plasticity induced by repetitive magnetic stimulation (rMS), a non-invasive brain stimulation technique used in clinical practice, is affected by LPS-induced inflammation. Specifically, we explored brain tissue cultures to learn more about the direct effects of LPS on neural tissue, and we tested for the plasticity-restoring effects of the anti-inflammatory cytokine interleukin 10 (IL10). As shown previously, 10 Hz repetitive magnetic stimulation (rMS) of organotypic entorhino-hippocampal tissue cultures induced a robust increase in excitatory neurotransmission onto CA1 pyramidal neurons. Furthermore, LPS-treated tissue cultures did not express rMS-induced synaptic plasticity. Live-cell microscopy in tissue cultures prepared from a novel transgenic reporter mouse line [ C57BL/6-Tg(TNFa-eGFP) ] confirms that ex vivo LPS administration triggers microglial tumor necrosis factor alpha (TNFα) expression, which is ameliorated in the presence of IL10. Consistent with this observation, IL10 hampers the LPS-induced increase in TNFα, IL6, IL1β, and IFNγ and restores the ability of neurons to express rMS-induced synaptic plasticity in the presence of LPS. These findings establish organotypic tissue cultures as a suitable model for studying inflammation-induced alterations in synaptic plasticity, thus providing a biological basis for the diagnostic use of transcranial magnetic stimulation in the context of brain inflammation.","journal":"Frontiers in Immunology","year":2020,"id":97495,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9473,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":482453,"name":"Amelie Eichler","orcid":"0000-0001-7990-654X","position":1,"is_corresponding":false},{"id":482454,"name":"Pia Kruse","orcid":"0000-0002-1742-1608","position":2,"is_corresponding":false},{"id":483701,"name":"Andreas Strehl","orcid":null,"position":3,"is_corresponding":false},{"id":482455,"name":"Silvia Rodriguez-Rozada","orcid":"0000-0001-6068-844X","position":4,"is_corresponding":false},{"id":482456,"name":"Itamar Goren","orcid":"0000-0001-5635-7963","position":5,"is_corresponding":false},{"id":482457,"name":"Nir Yogev","orcid":"0000-0002-9892-8581","position":6,"is_corresponding":false},{"id":482458,"name":"Stefan L. Frank","orcid":"0000-0002-7026-711X","position":7,"is_corresponding":false},{"id":297384,"name":"Ari Waisman","orcid":"0000-0003-4304-8234","position":8,"is_corresponding":false},{"id":469448,"name":"Thomas Deller","orcid":"0000-0002-3931-2947","position":9,"is_corresponding":false},{"id":124121,"name":"Steffen Jung","orcid":"0000-0003-4290-5716","position":10,"is_corresponding":false},{"id":482459,"name":"Nicola Maggio","orcid":"0000-0001-5041-2108","position":11,"is_corresponding":false},{"id":482460,"name":"Andreas Vlachos","orcid":"0000-0002-2646-3770","position":12,"is_corresponding":false},{"id":482452,"name":"Maximilian Lenz","orcid":"0000-0003-3147-4949","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-18T22:36:02.818637Z","pmid":"33391287","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}