{"doi":"10.3389/fimmu.2020.562138","title":"Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients","abstract":"Objective: Anti-DFS70 antibodies correlating with the nuclear dense fine speckled (DFS) pattern in the HEp-2 indirect immunofluorescence assay (IFA) are less common in patients with systemic autoimmune rheumatic disease (SARD) than in healthy subjects and their clinical associations remain elusive. We hosted a multi-center HEp-2 IFA training program to improve the ability of clinical laboratories to recognize the DFS pattern and to investigate the prevalence and relevance of anti-DFS70 antibodies. Methods: DFS pattern sera identified by HEp-2 IFA in 29 centers in China were redirected to a central laboratory for anti-DFS70 testing by line immunoblot assay (LIA), enzyme-linked immunosorbent assay (ELISA), and IFA with HEp-2 ELITE/DFS70-KO substrate. Anti-extractable nuclear antigen antibodies were measured by LIA and the clinical relevance was examined in adult and pediatric patients. Results: HEp-2 IFA positive rate and DFS pattern in positive sera were 36.2% (34,417/95,131) and 1.7% (582/34,417) in the patient cohort, and 10.0% (423/4,234) and 7.8% (33/423) in a healthy population, respectively. Anti-DFS70 prevalence among sera presenting the DFS pattern was 96.0, 93.7, and 49.6% by ELISA, LIA, and HEp-2 ELITE, respectively. 15.5% (52/336) of adult and 50.0% (20/40) of pediatric anti-DFS70 positive patients were diagnosed with SARD. Diseases most common in anti-DFS70 positive patients were spontaneous abortion (28.0%) in adults and juvenile idiopathic arthritis (22.5%) in pediatric patients. Conclusion: Accurate DFS pattern identification increased the detection rate of anti-DFS70 antibodies by ELISA and LIA. Anti-DFS70 antibodies are remarkably high in cases of spontaneous abortion and in pediatric SARD patients, but not prevalent in adult SARD patients.","journal":"Frontiers in Immunology","year":2020,"id":71198,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9577,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":377694,"name":"Zhiqing Wang","orcid":null,"position":1,"is_corresponding":false},{"id":376465,"name":"Rodrígo Mora","orcid":"0000-0001-7964-3575","position":2,"is_corresponding":false},{"id":376466,"name":"Aiping Liu","orcid":"0000-0001-8849-5228","position":3,"is_corresponding":false},{"id":377695,"name":"Chihui Li","orcid":null,"position":4,"is_corresponding":false},{"id":377696,"name":"Dengtao Liu","orcid":null,"position":5,"is_corresponding":false},{"id":377697,"name":"Fuying Zhai","orcid":null,"position":6,"is_corresponding":false},{"id":376467,"name":"Huiyuan Liu","orcid":"0000-0001-7206-6357","position":7,"is_corresponding":false},{"id":377698,"name":"Huiyun Gong","orcid":null,"position":8,"is_corresponding":false},{"id":377699,"name":"Jiaye Zhou","orcid":null,"position":9,"is_corresponding":false},{"id":376468,"name":"Jing Liu","orcid":"0000-0001-5394-1638","position":10,"is_corresponding":false},{"id":376469,"name":"Li Chen","orcid":"0000-0001-8492-1963","position":11,"is_corresponding":false},{"id":376470,"name":"Lijun Wu","orcid":"0000-0001-5876-8379","position":12,"is_corresponding":false},{"id":376471,"name":"Lin Yuan","orcid":"0000-0001-6966-8584","position":13,"is_corresponding":false},{"id":377700,"name":"Lina Ying","orcid":null,"position":14,"is_corresponding":false},{"id":377701,"name":"Loujian Jie","orcid":null,"position":15,"is_corresponding":false},{"id":377702,"name":"Meifang He","orcid":null,"position":16,"is_corresponding":false},{"id":376472,"name":"Meng Hao","orcid":"0009-0008-6489-9699","position":17,"is_corresponding":false},{"id":376473,"name":"Ping Xu","orcid":"0000-0002-4118-2598","position":18,"is_corresponding":false},{"id":377703,"name":"Qiuwei Lu","orcid":null,"position":19,"is_corresponding":false},{"id":376474,"name":"Shanshan Han","orcid":"0000-0002-7382-6028","position":20,"is_corresponding":false},{"id":377704,"name":"Shishi Chen","orcid":null,"position":21,"is_corresponding":false},{"id":377705,"name":"Shuimian Chen","orcid":null,"position":22,"is_corresponding":false},{"id":377706,"name":"Shunfei Zhu","orcid":null,"position":23,"is_corresponding":false},{"id":376475,"name":"Weihua Sun","orcid":"0000-0003-4578-8113","position":24,"is_corresponding":false},{"id":376476,"name":"Xiaoying Guo","orcid":"0000-0002-9068-5650","position":25,"is_corresponding":false},{"id":376477,"name":"Yajuan Chen","orcid":"0000-0002-5781-4959","position":26,"is_corresponding":false},{"id":376478,"name":"Yan Wang","orcid":"0000-0002-6511-1495","position":27,"is_corresponding":false},{"id":376479,"name":"Ye-Min Qu","orcid":"0000-0002-6212-3669","position":28,"is_corresponding":false},{"id":376480,"name":"Zhen Li","orcid":"0000-0003-0333-7699","position":29,"is_corresponding":false},{"id":377707,"name":"Zhenzhen Niu","orcid":null,"position":30,"is_corresponding":false},{"id":376481,"name":"Zhongyan Han","orcid":"0009-0005-8909-9426","position":31,"is_corresponding":false},{"id":247405,"name":"Edward K. 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