{"doi":"10.3389/fimmu.2020.02043","title":"Targeted Co-delivery of Tumor Antigen and α-Galactosylceramide to CD141+ Dendritic Cells Induces a Potent Tumor Antigen-Specific Human CD8+ T Cell Response in Human Immune System Mice","abstract":"Active co-delivery of tumor antigens (Ag) and α-galactosylceramide (α-GalCer), a potent agonist for invariant Natural Killer T (iNKT) cells, to cross-priming CD8α+ dendritic cells (DCs) was previously shown to promote strong anti-tumor responses in mice. Here, we designed a nanoparticle-based vaccine able to target human CD141+ (BDCA3+) DCs - the equivalent of murine CD8α+ DCs - and deliver both tumor Ag (Melan A) and α-GalCer. This nanovaccine was inoculated into humanized mice that mimic the human immune system (HIS) and possess functional iNKT cells and CD8+ T cells, called HIS-CD8/NKT mice. We found that multiple immunizations of HIS-CD8/NKT mice with the nanovaccine resulted in the activation and/or expansion of human CD141+ DCs and iNKT cells and ultimately elicited a potent Melan-A-specific CD8+ T cell response, as determined by tetramer staining and ELISpot assay. Single-cell proteomics further detailed the highly polyfunctional CD8+ T cells induced by the nanovaccine and revealed their predictive potential for vaccine potency. This finding demonstrates for the first time the unique ability of human iNKT cells to license cross-priming DCs in vivo and adds a new dimension to the current strategy of cancer vaccine development.","journal":"Frontiers in Immunology","year":2020,"id":102539,"datarank":0.8856492916302843,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.39127376172963485,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.39127376172963485,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":21,"citers_with_citation_signal":17,"citers_with_endowment":17,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9508,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":109714,"name":"Jing Zhou","orcid":"0000-0001-6190-8053","position":1,"is_corresponding":false},{"id":501882,"name":"Reem Ghinnagow","orcid":null,"position":2,"is_corresponding":false},{"id":500706,"name":"Toshiyuki Seki","orcid":"0000-0002-7559-7089","position":3,"is_corresponding":false},{"id":350800,"name":"Sho Iketani","orcid":"0000-0002-3733-9556","position":4,"is_corresponding":false},{"id":501883,"name":"Daphnée Soulard","orcid":null,"position":5,"is_corresponding":false},{"id":501884,"name":"Patrick Paczkowski","orcid":null,"position":6,"is_corresponding":false},{"id":500707,"name":"Yukiko Tsuji","orcid":"0000-0002-4570-6125","position":7,"is_corresponding":false},{"id":109727,"name":"Sean Mackay","orcid":"0009-0006-4030-916X","position":8,"is_corresponding":false},{"id":500708,"name":"Luis J. Cruz","orcid":"0000-0002-0802-1696","position":9,"is_corresponding":false},{"id":310814,"name":"François Trottein","orcid":"0000-0003-3373-1814","position":10,"is_corresponding":false},{"id":500709,"name":"Moriya Tsuji","orcid":"0000-0001-8587-277X","position":11,"is_corresponding":false},{"id":500705,"name":"Jing Huang","orcid":"0000-0002-0638-4805","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T22:41:53.173268Z","pmid":"32973811","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}