{"doi":"10.3389/fimmu.2020.01848","title":"Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon","abstract":"The cell wall of wild-type (WT) Mycobacterium tuberculosis (Mtb), an etiologic agent of tuberculosis (TB) and a Mtb strain disrupted in a 13-gene operon mce1 (Δmce1) varies by more than 400 lipid species. Here, we examined Mtb lipid-induced response in murine macrophage, as well as in human T-cell subpopulations in order to gain an insight into how changes in cell wall lipid composition may modulate host immune response. Relative to WT Mtb cell wall lipids, the non-polar lipid extracts from Δmce1 enhanced the mRNA expression of lipid-sense nuclear receptors TR4 and PPAR-γ and dampened the macrophage expression of genes encoding TNF-α, IL-6 and IL-1β. Relative to untreated control, WT lipid-pre-stimulated macrophages from healthy individuals induced a higher level of CD4-CD8- double negative T-cells (DN T-cells) producing TNF-α. Conversely, compared to WT, stimulation with Δmce1 lipids induced higher mean fluorescence intensity (MFI) in IL-10-producing DN T cells. Mononuclear cells from TB patients stimulated with WT Mtb lipids induced an increased production of TNF-α by CD8+ lymphocytes. Taken together, these observations suggest that changes in mce1 operon expression during a course of infection may serve as a strategy by Mtb to evade the host proinflammatory responses.","journal":"Frontiers in Immunology","year":2020,"id":83596,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":431190,"name":"Igor Müller","orcid":null,"position":1,"is_corresponding":false},{"id":430099,"name":"Luana E. Araújo","orcid":"0000-0002-7603-9396","position":2,"is_corresponding":false},{"id":430100,"name":"Thiago M. Cardoso","orcid":"0000-0002-0074-9789","position":3,"is_corresponding":false},{"id":416443,"name":"Lucas P. Carvalho","orcid":"0000-0001-5033-1666","position":4,"is_corresponding":false},{"id":431191,"name":"Bruna C. Barros","orcid":null,"position":5,"is_corresponding":false},{"id":430101,"name":"Maurício Brito Teixeira","orcid":"0000-0001-5828-9378","position":6,"is_corresponding":false},{"id":416444,"name":"Sérgio Arruda","orcid":"0000-0002-3071-6339","position":7,"is_corresponding":false},{"id":431192,"name":"Lee W. Riley","orcid":null,"position":8,"is_corresponding":false},{"id":431193,"name":"Adriano Queiroz","orcid":null,"position":9,"is_corresponding":false},{"id":431189,"name":"Jéssica Dias Petrilli","orcid":null,"position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T21:54:32.422022Z","pmid":"32973761","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}